Effects of Interfacial Properties of a Surface Modified Surface Plasmon Resonance Chip on Protein Immobilization Performance

被引:0
|
作者
Juan Wu
Yanbin Jiang
Lishi Wang
Qiang Zeng
机构
[1] South China University of Technology,School of Chemistry and Chemical Engineering
[2] South China University of Technology,State Key Laboratory of Pulp and Paper Engineering
来源
Analytical Sciences | 2017年 / 33卷
关键词
Surface plasmon resonance array; surface modification; protein immobilization; nonspecific adsorption;
D O I
暂无
中图分类号
学科分类号
摘要
In order to confirm the correlation between interfacial properties of modified surface plasmon resonance (SPR) chips and their SPR responses to immobilized anti-IgG, SPR chips were modified by mercaptoundecanoic acid, poly(ethylene glycol) diacrylate (PEG), PEG-based copolymer and cyclodextrin coupled PEG using self-assembled or radical polymerization methods. The resulting interfacial properties such as film thickness and hydrophilicity were characterized by AFM, elliptic polarization scanners and contact angle meter. Immobilization of human IgG on the modified chips was achieved by EDC/NHS activation through an amide bond. The association between fixed IgG and free anti-IgG was reflected by the variation of SPR responses and the binding ability was evaluated by Langmuir isotherms. As observed, the adsorption between IgG and anti-IgG was affected by the interfacial properties of different modifiers, such that a chip with a thinner and more hydrophilic layer may result in a higher SPR response, producing a larger adsorption equilibrium constant for protein interaction.
引用
收藏
页码:481 / 485
页数:4
相关论文
共 50 条
  • [21] IMMOBILIZATION OF PROTEINS TO A CARBOXYMETHYLDEXTRAN-MODIFIED GOLD SURFACE FOR BIOSPECIFIC INTERACTION ANALYSIS IN SURFACE-PLASMON RESONANCE SENSORS
    JOHNSSON, B
    LOFAS, S
    LINDQUIST, G
    ANALYTICAL BIOCHEMISTRY, 1991, 198 (02) : 268 - 277
  • [22] Development of protein chip based on surface plasmon resonance for the detection of Actinobacillus pleuropneumoniae antibody
    Kim, Tae Jung
    Cho, Ho Seong
    Lee, Jae Il
    Park, Nam Yong
    JOURNAL OF RAPID METHODS AND AUTOMATION IN MICROBIOLOGY, 2007, 15 (02): : 206 - 215
  • [23] A novel method of immobilization of 911 for surface plasmon resonance biosensor
    Li, FC
    Qin, X
    Geng, MY
    CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 2003, 31 (03) : 266 - 270
  • [24] A Performance-Enhanced Bimetallic Chip for the Detection of Cadmium Ions with Surface Plasmon Resonance
    Pang, Kai
    Dong, Wei
    Zhang, Bing
    Zhan, Shuyue
    Wang, Xiaoping
    PLASMONICS, 2016, 11 (04) : 1119 - 1128
  • [25] A Performance-Enhanced Bimetallic Chip for the Detection of Cadmium Ions with Surface Plasmon Resonance
    Kai Pang
    Wei Dong
    Bing Zhang
    Shuyue Zhan
    Xiaoping Wang
    Plasmonics, 2016, 11 : 1119 - 1128
  • [26] Strategies for the Immobilization of Bacteriophages on Gold Surfaces Monitored by Surface Plasmon Resonance and Surface Morphology
    Tawil, Nancy
    Sacher, Edward
    Mandeville, Rosemonde
    Meunier, Michel
    JOURNAL OF PHYSICAL CHEMISTRY C, 2013, 117 (13): : 6686 - 6691
  • [27] Correlation of surface plasmon resonance and scanning probe microscopy for the study of protein immobilization and bioactivity at surfaces
    Davies, M.C.
    Dawkes, A.C.
    Shard, A.
    Leggett, G.J.
    Roberts, C.R.
    Davies, J.
    Shakesheff, K.
    Wilkins, M.
    Jackson, D.E.
    Tendler, S.J.B.
    Polymer Preprints, Division of Polymer Chemistry, American Chemical Society, 1993, 34 (02):
  • [28] PROTEIN IMMOBILIZATION ONTO IPA INTERFACE WITH OPTICAL FIBER SENSOR BASED ON SURFACE PLASMON RESONANCE
    Chen, Liang-Yu
    Tsao, Yu-Chia
    Lin, Yu-Cheng
    MICROWAVE AND OPTICAL TECHNOLOGY LETTERS, 2014, 56 (12) : 2826 - 2828
  • [29] Surface plasmon resonance as a probe of protein isomerization
    Winzor, DJ
    ANALYTICAL BIOCHEMISTRY, 2003, 318 (01) : 1 - 12
  • [30] Analysis of Protein Interactions by Surface Plasmon Resonance
    Drescher, Dennis G.
    Selvakumar, Dakshnamurthy
    Drescher, Marian J.
    PROTEIN-PROTEIN INTERACTIONS IN HUMAN DISEASE, PT A, 2018, 110 : 1 - 30