Phylogenetic diversity of Clavibacter michiganensis subsp. michiganensis isolates causing bacterial canker of tomato in Greece

被引:0
|
作者
Dimitris Malliarakis
Marianthi G. Pagoulatou
Evaggelia Mpalantinaki
Emmanouil Trantas
Filippos Ververidis
Dimitrios E. Goumas
机构
[1] Hellenic Mediterranean University,Laboratory of Biological and Biotechnological Applications, Department of Agriculture, School of Agricultural Sciences
[2] University Research Centre,Institute of Agri
[3] Hellenic Mediterranean University,Food and Life Sciences
来源
Journal of Plant Pathology | 2023年 / 105卷
关键词
Clavibacter michiganensis; Bacterial canker; Tomato; Phylogenetics; MLSA; Phytopathology; Crop disease;
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摘要
Tomato bacterial canker, caused by Clavibacter michiganensis subsp. michiganensis (Cmm) (Li et al. 2018), is a common destructive disease worldwide. The bacterium colonizes plant’s vascular system causing characteristic symptoms such as wilting, cankers, brown and black discoloration of vessels, white spots on the fruit and leaves. In the present work, isolates were collected from infected tomato plants of 11 Greek Prefectures during the years 2003-2018, and the genetic variability was investigated. After initial identification, 93 selected Cmm strains were subjected to phylogenetic analyses based on the sequencing of multiple loci (Multi-Locus Sequencing Analysis, MLSA) utilizing four housekeeping genes (atpD, ppk, kdpA and sdhA). MLSA highlighted 4.1% variability in nucleotide sequences and classified the 93 Cmm strains into 38 haplotypes. Nevertheless, the phylogenetic grouping of the strains was not in accordance with the geographical distribution, a fact that probably advocates the introduction of the disease from infected propagating material. However, strains isolated from the same area in a different year were placed in the same group which means that the pathogen can remain active between growing seasons. The study provides important epidemiological information on the disease outbreaks and the spread of Cmm in Greece.
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页码:1403 / 1419
页数:16
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