Cloning and characterisation of the adenosyl phosphosulphate kinase gene from Aspergillus nidulans

被引：0
|
作者：
David L. Clarke
Roger W. Newbert
G. Turner
机构：
[1] Department of Molecular Biology and Biotechnology,
[2] University of Sheffield,undefined
[3] Sheffield,undefined
[4] South Yorkshire,undefined
[5] S10 2UH,undefined
[6] UK Fax: ++44-114-2728697 e-mail: G.Turner@Sheffield.ac.uk,undefined
[7] Barton Laboratory,undefined
[8] Department of Plant Pathology,undefined
[9] Cornell University,undefined
[10] Geneva,undefined
[11] NY,undefined
[12] 14456,undefined
[13] USA,undefined
来源：
Current Genetics | 1997年 / 32卷
关键词：
Key words APS kinase; ATP sulphurylase; Aspergillus nidulans; Allosteric inhibition;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The sD gene of Aspergillus nidulans has been cloned by heterologous screening of rationally selected cosmids. Co-transformation of the sD50 mutant JMP1 confirmed the presence of a functional gene. Sequence analysis determined this gene to be 680 bp in length, containing a 59-bp intron and encoding a protein of 206 amino acids. A protein-sequence comparison revealed a similarity to the C-terminal region of ATP sulphurylase, the sC gene product. Further sequence comparison revealed differences in a consensus sequence ATP-binding motif, indicating non-functionality of the APS kinase-like domain of ATP sulphurylase, and confirms sD as the gene encoding APS kinase in A. nidulans.
引用
收藏
页码：408 / 412
页数：4
相关论文
共 50 条
  • [21] Cloning and characterization of an ERG27 like gene in Aspergillus nidulans
    Prakash, CCK
    Mirabito, PM
    MOLECULAR BIOLOGY OF THE CELL, 2002, 13 : 156A - 156A
  • [22] The metG gene of Aspergillus nidulans encoding cystathionine β-lyase:: cloning and analysis
    Sienko, M
    Paszewski, A
    CURRENT GENETICS, 1999, 35 (06) : 638 - 646
  • [23] Cloning and characterization of the Aspergillus nidulans cysB gene encoding cysteine synthase
    Jacek Topczewski
    Marzena Sienko
    A. Paszewski
    Current Genetics, 1997, 31 : 348 - 356
  • [24] INSERTIONAL INACTIVATION AND CLONING OF THE WA-GENE OF ASPERGILLUS-NIDULANS
    TILBURN, J
    ROUSSEL, F
    SCAZZOCCHIO, C
    GENETICS, 1990, 126 (01) : 81 - 90
  • [25] Cloning and characterization of the Aspergillus nidulans cysB gene encoding cysteine synthase
    Topczewski, J
    Sienko, M
    Paszewski, A
    CURRENT GENETICS, 1997, 31 (04) : 348 - 356
  • [26] CLONING AND CHARACTERIZATION OF THE CYTOCHROME-C GENE OF ASPERGILLUS-NIDULANS
    RAITT, DC
    BRADSHAW, RE
    PILLAR, TM
    MOLECULAR & GENERAL GENETICS, 1994, 242 (01): : 17 - 22
  • [27] Cloning and characterization of the gene encoding Aspergillus nidulans DNA topoisomerase II
    Kim, KH
    Akashi, T
    Mizuguchi, I
    Kikuchi, A
    GENE, 1999, 236 (02) : 293 - 301
  • [28] Identification and molecular cloning of a gene encoding Phospholipase A2 (plaA) from Aspergillus nidulans
    Hong, S
    Horiuchi, H
    Ohta, A
    BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 2005, 1735 (03): : 222 - 229
  • [29] Molecular cloning of a phospholipase D gene from Aspergillus nidulans and characterization of its deletion mutants
    Hong, S
    Horiuchi, H
    Ohta, A
    FEMS MICROBIOLOGY LETTERS, 2003, 224 (02) : 231 - 237
  • [30] Cloning and characterization of the nagA gene that encodes β-N-acetylglucosaminidase from Aspergillus nidulans and its expression in Aspergillus oryzae
    Kim, S
    Matsuo, I
    Ajisaka, K
    Nakajima, H
    Kitamoto, K
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2002, 66 (10) : 2168 - 2175
12345