Cloning and characterisation of the adenosyl phosphosulphate kinase gene from Aspergillus nidulans

被引：0

作者：

David L. Clarke

Roger W. Newbert

G. Turner

机构：

[1] Department of Molecular Biology and Biotechnology,

[2] University of Sheffield,undefined

[3] Sheffield,undefined

[4] South Yorkshire,undefined

[5] S10 2UH,undefined

[6] UK Fax: ++44-114-2728697 e-mail: G.Turner@Sheffield.ac.uk,undefined

[7] Barton Laboratory,undefined

[8] Department of Plant Pathology,undefined

[9] Cornell University,undefined

[10] Geneva,undefined

[11] NY,undefined

[12] 14456,undefined

[13] USA,undefined

来源：

Current Genetics | 1997年 / 32卷

关键词：

Key words APS kinase; ATP sulphurylase; Aspergillus nidulans; Allosteric inhibition;

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学科分类号：

摘要：

The sD gene of Aspergillus nidulans has been cloned by heterologous screening of rationally selected cosmids. Co-transformation of the sD50 mutant JMP1 confirmed the presence of a functional gene. Sequence analysis determined this gene to be 680 bp in length, containing a 59-bp intron and encoding a protein of 206 amino acids. A protein-sequence comparison revealed a similarity to the C-terminal region of ATP sulphurylase, the sC gene product. Further sequence comparison revealed differences in a consensus sequence ATP-binding motif, indicating non-functionality of the APS kinase-like domain of ATP sulphurylase, and confirms sD as the gene encoding APS kinase in A. nidulans.

引用

页码：408 / 412

页数：4