Cloning and characterisation of the adenosyl phosphosulphate kinase gene from Aspergillus nidulans

被引：0
|
作者：
David L. Clarke
Roger W. Newbert
G. Turner
机构：
[1] Department of Molecular Biology and Biotechnology,
[2] University of Sheffield,undefined
[3] Sheffield,undefined
[4] South Yorkshire,undefined
[5] S10 2UH,undefined
[6] UK Fax: ++44-114-2728697 e-mail: G.Turner@Sheffield.ac.uk,undefined
[7] Barton Laboratory,undefined
[8] Department of Plant Pathology,undefined
[9] Cornell University,undefined
[10] Geneva,undefined
[11] NY,undefined
[12] 14456,undefined
[13] USA,undefined
来源：
Current Genetics | 1997年 / 32卷
关键词：
Key words APS kinase; ATP sulphurylase; Aspergillus nidulans; Allosteric inhibition;
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学科分类号：
摘要：
The sD gene of Aspergillus nidulans has been cloned by heterologous screening of rationally selected cosmids. Co-transformation of the sD50 mutant JMP1 confirmed the presence of a functional gene. Sequence analysis determined this gene to be 680 bp in length, containing a 59-bp intron and encoding a protein of 206 amino acids. A protein-sequence comparison revealed a similarity to the C-terminal region of ATP sulphurylase, the sC gene product. Further sequence comparison revealed differences in a consensus sequence ATP-binding motif, indicating non-functionality of the APS kinase-like domain of ATP sulphurylase, and confirms sD as the gene encoding APS kinase in A. nidulans.
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页码：408 / 412
页数：4
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