Clinical evaluation of a panel of multiplex quantitative real-time reverse transcription polymerase chain reaction assays for the detection of 16 respiratory viruses associated with community-acquired pneumonia

被引：0
作者：
Dan Zhang
Haiyan Mao
Xiuyu Lou
Junhang Pan
Hao Yan
Hongfeng Tang
Yan Shu
Yun Zhao
Xiaoli Cheng
Hong Tao
Yanjun Zhang
Xuejun Ma
机构：
[1] Chinese Center for Disease Control and Prevention,Key Laboratory for Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention
[2] Institute of Microbiology,Department of Pathology
[3] Zhejiang Provincial Center for Disease Control and Prevention,Department of Pathology, School of Basic Medicine
[4] Children’s Hospital,undefined
[5] Zhejiang University School of Medicine,undefined
[6] Hubei University of Medicine,undefined
[7] Department of Rheumatology and Immunology Renmin Hospital,undefined
[8] Hubei University of Medicine,undefined
来源：
Archives of Virology | 2018年 / 163卷
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摘要：
We developed a panel of multiplex quantitative real-time reverse transcription polymerase chain reaction (mqRT-PCR) assay consisting of seven internally controlled qRT-PCR assays to detect 16 different respiratory viruses. We compared the new mqRT-PCR with a previously reported two-tube mRT-PCR assay using 363 clinical sputum specimens. The mqRT-PCR assay performed comparably with the two-tube assay for most viruses, offering the advantages of quantitative analysis, easier performance, lower susceptibility to contamination, and shorter turnaround time in laboratories equipped with conventional real-time PCR instrumentation, and it could therefore be a valuable tool for routine surveillance of respiratory virus infections in China.
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页码：2855 / 2860
页数：5
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