Transforming growth factor-β2 inhibits interleukin 1β-induced expression of inducible nitric oxide synthase in rat renal mesangial cells

被引：0

作者：

D. Kunz

G. Walker

J. Pfeilschifter

机构：

[1] Department of Pharmacology,

[2] Biozentrum,undefined

[3] University of Basel,undefined

[4] Klingelbergstr. 70,undefined

[5] CH-4056 Basel,undefined

[6] Switzerland,undefined

[7] Zentrum der Pharmakologie,undefined

[8] Klinikum der Johann-Wolfgang-Goethe-Universität,undefined

[9] Theodor-Stern-Kai 7,undefined

[10] D-60590 Frankfurt am Main,undefined

[11] Germany,undefined

[12] Fax +49 69 6301 7942,undefined

来源：

Inflammation Research | 1997年 / 46卷

关键词：

Key words: Nitric oxide — Interleukin-1β— Transforming growth factor β— Mesangial cell — Nitric oxide synthase;

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摘要：

Objective and Design: We have investigated whether transforming growth factor β2 (TGF-β2) influences the expression of inducible nitric oxide synthase (iNOS).¶Material: Rat renal mesangial cells exposed to the inflammatory cytokine interleukin 1β (IL-1β).¶Results: Addition of TGF-β2 dose-dependently suppresses IL-1β-induced nitrite formation. Western and Northern blot analyses of mesangial cell extracts reveal that the inhibition of IL-1β-induced nitrite formation by TGF-β2 is due to decreased iNOS protein and iNOS mRNA steady state levels. Reduction of iNOS mRNA levels is due to decreased transcriptional activity of the iNOS gene under the action of TGF-β2 as shown by nuclear run on experiments.¶Conclusions: TGF-β2 is a potent inhibitor of iNOS expression acting by reducing the transcriptional activity of the iNOS gene.

引用

页码：327 / 331

页数：4