Oxidative stress on DNA in chronic renal failure: The influence of different hemodialysis membranes

被引：0

作者：

Kuragano T. ^{[1
]}

Kuno T. ^{[1
]}

Yamamoto C. ^{[1
]}

Nagura Y. ^{[1
]}

Takahashi S. ^{[1
]}

Kanmatsuse K. ^{[1
]}

机构：

[1] Second Dept. of Internal Medecine, Nihon University School of Medicine, Itabashi-ku, Tokyo 173-8610

来源：

Journal of Artificial Organs | 2001年 / 4卷 / 4期

关键词：

8-OHdG; Chronic renal disease; Hemodialysis; Oxidative stress on DNA;

D O I：

10.1007/BF02480025

中图分类号：

学科分类号：

摘要：

It has been reported that uremia is a state of oxidative stress and may play an important role as a pathological cause of various uremic complications. Oxidative stress is known to increase conversion of deoxyguanosine to 8-hydroxy-2-deoxyguanosine (8-OHdG) in DNA, and 8-OHdG is used as a marker of oxidative DNA damage. We evaluated plasma and urinary concentrations of 8-OHdG in 49 patients (male 28, female 21; mean age 65 years; diabetic 27, nondiabetic 22) with chronic renal disease (CRD) and 22 patients (male 14, female 8; mean age 63 year; diabetic 7, nondiabetic 15) on maintenance hemodialysis (M-HD). Plasma concentrations of 8-OHdG were measured using a highly sensitive ELISA kit, and the urinary mean concentrations of 8-OHdG were measured using an ELISA kit. Plasma concentrations of creatinine (Cr), Urea nitrogen (UN), and β2-microgloblin (β2-MG) and 24-h creatinine clearance (CCr) were also measured in CRD patients. Furthermore, 8-OHdG was measured before the dialysis session in M-HD patients. The plasma concentration of 8-OHdG in patients on CRD was significantly correlated with serum-creatinine (S-Cr), serum-umea nitrogen (S-UN), and β2-MG (P < 0.0001) and also significantly negatively correlated with CCr (P < 0.005), but was not significantly correlated with age, fasting blood suger (FBS), hemoglobin A1C (HbA1C), and urinary concentration of 8-OHdG were not correlated with S-Cr, S-UN, β2-MG, and CCr. The plasma mean concentrations of 8-OHdG in patients on CRD and M-HD were as follows: CRD (CCr > 50ml/min, n = 12), 0.108 ± 0.41 ng/ml; CRD (CCr < 10ml/min,n = 9), 0.277 ± 0.15 ng/ml; M-HD (n = 22), 0.217 ± 0.59ng/ml (mean ± SD). The mean plasma concentration of 8-OHdG was 0.296 ± 0.75 ng/ml in patients on M-HD in the polysulfone membrane group, 0.304 ± 0.122ng/ml their cellulose membrane group, and 0.354 ± 0.21ng/ml their vitamin E-modified cellulose membrane group. This study showed that in CRD patients, oxidative stress on DNA increasesed with the progression of renal disease, and that end-stage CRD patients were already exposed to the same degree of oxidative stress on DNA as M-HD patients. In M-HD patients, oxidative stress on DNA was not related to the type of hemodialysis membrane.

引用

页码：320 / 325

页数：5

共 21 条

[1]

Hasselwandor O., Young I.S., Oxidative stress in chronic renal failure, Free Radic Res, 29, pp. 1-11, (1998)

[2]

Canaud B., Cristol J.-P., Morena M., Leray-Moragues H., Bosc J.-Y., Imbalance of oxidants and anti oxidants in hemodialysis patients, Blood Purif, 17, pp. 99-106, (1999)

[3]

Tetta C., Biasioli S., Schiavon R., Inguaggiato P., David S., Panichi V., Wratten M.L., An overview of hemodialysis and oxidant stress, Blood Purif, 17, pp. 118-126, (1999)

[4]

Galli F., Ronco C., Oxidants stress in hemodialysis, Nephron, 84, pp. 1-15, (2000)

[5]

Galli F., Canestrari F., Buoncristiani U., Biological effects of oxidants stress in hemodialysis

[6]

the possible role of vitamin E, Blood Purif, 17, pp. 79-94, (1999)

[7]

Shigenaga M., Gimeno C., Ames B., Urinary 8-hydroxy-2-deoxyguanosine as a biological marker of in vivo oxidative DNA damage, Proc Natl Acad Sci USA, 86, pp. 9697-9701, (1989)

[8]

Hinokio Y., Suzuki S., Hirai M., Chiba M., Hirai A., Toyota T., Oxidative DNA damage in diabetes mellitus: Its association with diabetes complications, Diabetologic, 42, pp. 995-998, (1994)

[9]

Suzuki S., Hinokio Y., Komatu K., Ohtomo M., Onoda M., Hirai S., Hirai A., Chiba M., Kasuga S., Akai H., Toyota T., Oxidative damage to mitochondrial DNA and its relationship to diabetic complications, Diabetes Res Clin Pract, 45, pp. 161-168, (1999)

[10]

Cooke M.S., Evans M.D., Podmore I.D., Herbert K.E., Mistry N., Mistry D., Hickenbotham P.T., Hussieni A., Griffiths H.R., Lunec J., Novel repair action of vitamin C upon in vivo oxidative DNA damage, FEBS Lett, 363, pp. 363-367, (1998)

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