Sulfite increases lipoperoxidation and decreases the activity of catalase in brain of rats

被引：0
作者：
Fábria Chiarani
Caren S. Bavaresco
Carlos S. Dutra-Filho
Carlos Alexandre Netto
Angela T. S. Wyse
机构：
[1] Universidade Federal do Rio Grande do Sul,Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde
来源：
Metabolic Brain Disease | 2008年 / 23卷
关键词：
Sulfite oxidase deficiency; Metabolic disease; Sulfite; Oxidative stress;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The main objective of this study was to investigate the in vitro effects of sulfite, a metabolite accumulated in isolated sulfite oxidase deficiency, on Na +, K +-ATPase activity and on some parameters of oxidative stress, namely thiobarbituric acid-reactive substances (TBARS) and catalase activity (antioxidant enzyme) in cerebral cortex, striatum and hippocampus from 10- and 60-day-old rats. Results showed that 500 μM sulfite significantly increased TBARS and reduced catalase activity in the cerebral structures studied from neonates and adults rats; in contrast, sulfite did not alter Na+, K+-ATPase activity. Our present findings show that sulfite increases lipid peroxidation and decreases antioxidant enzyme defenses in rat brain, suggesting an induction of oxidative stress. We presumed that oxidative stress might be, at least in part, associated with the neuronal dysfunction of patients affected by isolated sulfite oxidase deficiency.
引用
收藏
页码：123 / 132
页数：9
相关论文
共 125 条
  • [1] Aebi H(1984)Catalase in vitro Methods Enzymol 105 121-126
  • [2] Baker MT(2002)Sulfite supported lipid peroxidation in Propofol emulsions Anesthesiology 97 1162-1167
  • [3] Dehring DJ(1976)A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein–die binding Anal Biochem 72 248-254
  • [4] Gregerson MS(1986)A direct colorimetric assay for Ca2+-stimulated ATPase activity Anal Biochem 157 375-380
  • [5] Bradford MM(2002)Infantile isolated sulphite oxidase deficiency in a Chinese family: a rare neurodegenerative disorder Hong Kong Med J 8 279-282
  • [6] Chan KM(2006)Effect of sodium sulfite on mast cell degranulation and oxidant stress Ann Allergy Asthma & Immunol 96 550-556
  • [7] Delfert D(2001)Effects of methyl mercury, mercuric sulfide and cinnabar on active avoidance responses, Na+ /K+-ATPase activities and tissue mercury contents in rats Proc Natl Sci Counc Repub China Part B 25 128–136-240
  • [8] Junger KD(2006)The effect of sulfite and chronic restraint stress on brain lipid peroxidation and anti-oxidant enzyme activities Toxicol Ind Health 22 233-1961
  • [9] Chan K(1999)Isolated sulfite oxidase deficiency: Review of two cases in one family Ophthalmology 106 1957-82
  • [10] Li C(2005)The prooxidant effect of sodium metabisulfite in rat liver and kidney Regul Toxicol Pharmacol 42 77-29
12345678910