Effect of secreted Rpf protein on intracellular contacts in Micrococcus luteus and Mycobacterium smegmatis cultures

被引：0
|
作者：
V. D. Nikitushkin
G. R. Demina
A. S. Kaprelyants
机构：
[1] Russian Academy of Sciences,Bakh Institute of Biochemistry
来源：
Microbiology | 2011年 / 80卷
关键词：
biofilms; resuscitation promoting factor (Rpf); nitrophenylthiocyanate;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The effect of Resuscitation promoting factor (Rpf) on intercellular contacts in the cultures of Micrococcus luteus and Mycobacterium smegmatis was investigated using dynamic light scattering (DLS, photon correlation spectroscopy). During the stationary growth phase, the cells of the tested cultures formed extensive aggregates 100 and 300 μ in size for M. smegmatis and M. luteus, respectively. The number of solitary cells was insignificant. Addition of the recombinant Rpf protein (15 μg/ml) resulted dispersion of cell aggregates and emergence of solitary cells. This effect of Rpf decreased in the presence of nitrophenylthiocyanates (NPTs), specific Rpf inhibitors. Presumably, Rpf is involved in the regulation of intracellular interactions and in biofilm formation.
引用
收藏
相关论文
共 23 条
  • [1] Effect of secreted Rpf protein on intracellular contacts in Micrococcus luteus and Mycobacterium smegmatis cultures
    Nikitushkin, V. D.
    Demina, G. R.
    Kaprelyants, A. S.
    MICROBIOLOGY, 2011, 80 (02) : 143 - 149
  • [2] The rpf gene of Micrococcus luteus encodes an essential secreted growth factor
    Mukamolova, GV
    Turapov, OA
    Kazarian, K
    Telkov, M
    Kaprelyants, AS
    Kell, DB
    Young, M
    MOLECULAR MICROBIOLOGY, 2002, 46 (03) : 611 - 621
  • [3] Evaluation of Rpf protein of Micrococcus luteus for cultivation of soil actinobacteria
    Wang, Yuhui
    Shi, Jiangli
    Tang, Lingjie
    Zhang, Yufan
    Zhang, Yujia
    Wang, Xinyu
    Zhang, Xiumin
    SYSTEMATIC AND APPLIED MICROBIOLOGY, 2021, 44 (05)
  • [4] Involvement of the Mycobacterium tuberculosis secreted antigen SA-5K in intracellular survival of recombinant Mycobacterium smegmatis
    Batoni, G
    Bottai, D
    Maisetta, G
    Pardini, M
    Boschi, A
    Florio, W
    Esin, S
    Campa, M
    FEMS MICROBIOLOGY LETTERS, 2001, 205 (01) : 125 - 129
  • [5] Structural and Functional Insights into Mycobacterium tuberculosis and Mycobacterium smegmatis Enhanced intracellular survival protein
    Yoon, Hye-Jin
    Kim, Kyoung Hoon
    An, Doo Ri
    Yoon, Ji Young
    Kim, Hyoun Sook
    Im, Ha Na
    Kim, Jieun
    Suh, Se Won
    ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES, 2012, 68 : S165 - S165
  • [6] Interaction of Erp Protein of Mycobacterium tuberculosis with Rv2212 Enhances Intracellular Survival of Mycobacterium smegmatis
    Ganaie, Arsheed Ahmad
    Trivedi, Garima
    Kaur, Amanpreet
    Jha, Sidharth Shankar
    Anand, Shashi
    Rana, Vibhuti
    Singh, Amit
    Kumar, Shekhar
    Sharma, Charu
    JOURNAL OF BACTERIOLOGY, 2016, 198 (20) : 2841 - 2852
  • [7] Effect of Mycobacterium tuberculosis protein Rv0309 on intracellular survival of Mycobacterium smegmatis by inhibiting macrophage autophagy via protein STUB1
    李璐
    ChinaMedicalAbstracts(InternalMedicine), 2023, 40 (02) : 85 - 86
  • [8] The effect of methane on NADN-oxidase activity of Au-protein from Micrococcus luteus
    Levchenko, LA
    Sadkov, AP
    Lariontseva, NV
    Borod'ko, AY
    Kulikov, AV
    Shilov, AE
    DOKLADY AKADEMII NAUK, 1999, 364 (01) : 117 - 118
  • [9] Aminoglycoside Multiacetylating Activity of the Enhanced Intracellular Survival Protein from Mycobacterium smegmatis and Its Inhibition
    Chen, Wenjing
    Green, Keith D.
    Tsodikov, Oleg V.
    Garneau-Tsodikova, Sylvie
    BIOCHEMISTRY, 2012, 51 (24) : 4959 - 4967
  • [10] EFFECT OF IRON AND ZINC ON NUCLEIC ACID AND PROTEIN SYNTHESIS IN MYCOBACTERIUM-SMEGMATIS
    WINDER, F
    DENNENY, JM
    NATURE, 1959, 184 (4687) : 742 - 743
123