Immunocytochemical Detection of Anti-Hippocampal Antibodies in Alzheimer's Disease and Normal Cerebrospinal Fluid

被引:0
作者
David A. Loeffler
Paul L. Juneau
Hanh-Uyen Nguyen
Dina Najman
Nunzio Pomara
Peter A. LeWitt
机构
[1] Sinai Hospital,Neuroscience Program
[2] Nathan S. Kline Institute for Psychiatric Research,Division of Geriatric Psychiatry
[3] Wayne State University School of Medicine,Departments of Neurology and Psychiatry
来源
Neurochemical Research | 1997年 / 22卷
关键词
Alzheimer's disease; antibodies; cerebrospinal fluid; complement; hippocampus;
D O I
暂无
中图分类号
学科分类号
摘要
Immunocytochemical staining was performed to investigate the presence of anti-hippocampal antibodies in cerebrospinal fluid (CSF) from patients with probable Alzheimer's disease (AD) (n = 19), aged normal controls (n = 9), and young normal controls (n = 10). Marked staining of neurons in the granule cell layer of the dentate gyrus and in pyramidal neurons in CA1-3 of the rat hippocampus was observed in 5 AD CSF samples (26%), 1 aged control sample (11%), and 1 young control sample (10%). These differences were not statistically significant. One of the immunoreactive AD CSF specimens also contained high concentrations of C5b-9, the membrane attack complex. The infrequent occurrence of anti-hippocampal antibodies in AD CSF, and the detection of similar immunoreactivity in control CSF specimens, suggest that these antibodies are unlikely to play a role in the neurodegenerative process in most individuals with AD. However, elevated C5b-9 concentration in an AD CSF specimen with marked immunoreactivity to hippocampal neurons suggests the possibility that anti-neuronal antibodies may contribute to complement activation in some AD patients.
引用
收藏
页码:209 / 214
页数:5
相关论文
共 126 条
[1]  
Ball M. J.(1977)Neuronal loss, neurofibrillary tangle and granulovacuolar degeneration in the hippocampus with aging and dementia Acta Neuropathol. 37 111-118
[2]  
Hyman B. T.(1984)Alzheimer's disease: cell-specific pathology isolates the hippocampal formation Science 255 1168-1170
[3]  
Van Hoesen G. W.(1994)Neuroimmune mechanisms in Alzheimer disease pathogenesis Alz. Dis. Assoc. Disorders 8 149-158
[4]  
Damasio A. R.(1986)Detection of brain autoantibodies in the serum of patients with Alzheimer's disease but not Down's syndrome Immunol. Lett. 12 277-280
[5]  
Barnes C. L.(1988)Antibodies to cholinergic neurons in Alzheimer's disease J. Neurochem. 51 479-485
[6]  
McGeer P. L.(1989)Neuron-binding antibodies in Alzheimer's disease and Down's syndrome J. Gerontol. 44 M128-130
[7]  
Rogers J.(1987)Antibodies in cerebrospinal fluid of some Alzheimer disease patients recognize cholinergic neurons in the rat central nervous system Proc. Natl. Acad. Sci. U.S.A. 84 9214-9218
[8]  
McGeer E. G.(1988)Antibodies recognizing cholinergic neurons and thyroglobulin are found in the cerebrospinal fluid of a subgroup of patients with Alzheimer's disease Drug Dev. Res. 15 153-163
[9]  
Singh V. K.(1990)Investigations on auto-antibodies in Alzheimer's and Parkinson's diseases, using defined neuronal cultures J. Neural. Transm. 29 195-206
[10]  
Fudenberg H. H.(1991)Antibodies in the cerebrospinal fluid of some Alzheimer's disease patients recognize amoeboid microglial cells in the developing rat central nervous system Neurosci. 41 739-752