Next-generation sequencing for hypothesis-free genomic detection of invasive tropical infections in poly-microbially contaminated, formalin-fixed, paraffin-embedded tissue samples – a proof-of-principle assessment

[1] Mauch H(2008)Einfluss von Lagerung und Transport auf das Überleben von Infektionserregern. Bedeutung für eine schnelle mikrobiologische Diagnostik, die Ökonomie und den Patienten Mikrobiologe 18 200-204

[2] Hagen RM(2002)Strategies for PCR based detection of Mol Pathol 55 398-400

[3] Gauthier YP(2004) DNA in paraffin wax embedded tissues BMC Clin Pathol 4 1-5

[4] Sprague LD(2010)In vitro mutation artifacts after formalin fixation and error prone translesion synthesis during PCR BMC Res Notes 3 239-3399

[5] Vidal DR(2010)Comparative analysis of four methods to extract DNA from paraffin-embedded tissues: effect on downstream molecular applications J Am Chem Soc 132 3388-244

[6] Zysk G(2013)Structural characterization of formaldehyde-induced cross-links between amino acids and desoxynucleotides and their oligomers PLoS One 8 e77771-1444

[7] Finke EJ(2013)Improved PCR performance using template DNA from formalin-fixed and paraffin-embedded tissues by overcoming PCR inhibition Diagn Mol Pathol 22 236-S202

[8] Quach N(2005)Influence of parasite density and sample storage time on the reliability of Clin Infect Dis 41 1438-3128

[9] Goodman MF(2009)-specific PCR from formalin-fixed and paraffin-embedded tissues Electrophoresis 30 S196-528

[10] Shibata D(2015)Impact of rapid detection of viral and atypical bacterial pathogens by real-time polymerase chain reaction for patients with lower respiratory tract infection Eur Heart J 36 3075-1510

[1] Mauch H(2008)Einfluss von Lagerung und Transport auf das Überleben von Infektionserregern. Bedeutung für eine schnelle mikrobiologische Diagnostik, die Ökonomie und den Patienten Mikrobiologe 18 200-204

[2] Hagen RM(2002)Strategies for PCR based detection of Mol Pathol 55 398-400

[3] Gauthier YP(2004) DNA in paraffin wax embedded tissues BMC Clin Pathol 4 1-5

[4] Sprague LD(2010)In vitro mutation artifacts after formalin fixation and error prone translesion synthesis during PCR BMC Res Notes 3 239-3399

[5] Vidal DR(2010)Comparative analysis of four methods to extract DNA from paraffin-embedded tissues: effect on downstream molecular applications J Am Chem Soc 132 3388-244

[6] Zysk G(2013)Structural characterization of formaldehyde-induced cross-links between amino acids and desoxynucleotides and their oligomers PLoS One 8 e77771-1444

[7] Finke EJ(2013)Improved PCR performance using template DNA from formalin-fixed and paraffin-embedded tissues by overcoming PCR inhibition Diagn Mol Pathol 22 236-S202

[8] Quach N(2005)Influence of parasite density and sample storage time on the reliability of Clin Infect Dis 41 1438-3128

[9] Goodman MF(2009)-specific PCR from formalin-fixed and paraffin-embedded tissues Electrophoresis 30 S196-528

[10] Shibata D(2015)Impact of rapid detection of viral and atypical bacterial pathogens by real-time polymerase chain reaction for patients with lower respiratory tract infection Eur Heart J 36 3075-1510