Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes

被引：0
作者：
Sang H. Lee
Seung Min Shin
Peng Zhong
Hyun-Taek Kim
Dong-Il Kim
June Myoung Kim
Won Do Heo
Dae-Won Kim
Chang-Yeol Yeo
Cheol-Hee Kim
Qing-song Liu
机构：
[1] Medical College of Wisconsin,Department of Pharmacology and Toxicology
[2] Medical College of Wisconsin,Neuroscience Research Center
[3] Chungnam National University,Department of Biology
[4] Yonsei University,Department of Biochemistry
[5] Korea Advanced Institute of Science and Technology,Department of Biological Sciences
[6] Ewha Woman’s University,Department of Life Science and Research Center for Cellular Homeostasis
来源：
Nature Communications | / 9卷
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摘要：
Secreted Wnts play crucial roles in synaptogenesis and synapse maintenance, but endogenous factors promoting synapse elimination in central neurons remain unknown. Here we show that proline-rich 7 (PRR7) induces specific removal of excitatory synapses and acts as a Wnt inhibitor. Remarkably, transmembrane protein PRR7 is activity-dependently released by neurons via exosomes. Exosomal PRR7 is uptaken by neurons through membrane fusion and eliminates excitatory synapses in neighboring neurons. Conversely, PRR7 knockdown in sparse neurons greatly increases excitatory synapse numbers in all surrounding neurons. These non-cell autonomous effects of PRR7 are effectively negated by augmentation or blockade of Wnt signaling. PRR7 exerts its effect by blocking the exosomal secretion of Wnts, activation of GSK3β, and promoting proteasomal degradation of PSD proteins. These data uncover a proximity-dependent, reciprocal mechanism for the regulation of excitatory synapse numbers in local neurons and demonstrate the significance of exosomes in inter-neuronal signaling in the vertebrate brain.
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