Fecal Microbiota Transplantation Exerts a Protective Role in MPTP-Induced Parkinson’s Disease via the TLR4/PI3K/AKT/NF-κB Pathway Stimulated by α-Synuclein

被引:0
作者
Zhe Zhong
Weijie Chen
Huan Gao
Ningning Che
Min Xu
Lanqing Yang
Yingfang Zhang
Min Ye
机构
[1] Affiliated BenQ Hospital of Nanjing Medical University,Department of Neurology
来源
Neurochemical Research | 2021年 / 46卷
关键词
Gut microbiota; Parkinson's disease; α-synuclein; TLR4/PI3K/AKT/NF-κB signaling;
D O I
暂无
中图分类号
学科分类号
摘要
Gut microbiota is closely related to the Parkinson’s disease (PD) pathogenesis. Additionally, aggregation of α-synuclein (α-syn) is central to PD pathogenesis. Here we identified the further mechanisms of gut microbiota in PD. A mouse model with PD was established via injection of MPTP. Normal or MPTP-induced PD like animals were treated with FMT from healthy normal mice. Pole test and traction test were performed to examine the effects of FMT on motor function of PD mice. Fecal SCFAs were assessed by gas chromatography-mass spectrometry. The α-syn level in the substantia nigra pars compacta (SN) of mice was measured using western blot. Dopaminergic neurons and microglial activation in the SN were analyzed by immunohistochemistry (IHC) and immunofluorescence (IF) staining. FMT alleviated physical impairment, decreased fecal SCFAs in a mouse model of PD. Additionally, FMT decreased the expression of α-syn, as well as inhibited the activation of microglia in the SN, and blocked the TLR4/PI3K/AKT/NF-κB signaling in the SN and striatum. FMT could protect mice against PD via suppressing α-syn expression and inactivating the TLR4/PI3K/AKT/NF-κB signaling.
引用
收藏
页码:3050 / 3058
页数:8
相关论文
共 279 条
[51]  
Zhu YL(undefined)undefined undefined undefined undefined-undefined
[52]  
Zhou ZL(undefined)undefined undefined undefined undefined-undefined
[53]  
Jia XB(undefined)undefined undefined undefined undefined-undefined
[54]  
Xu YD(undefined)undefined undefined undefined undefined-undefined
[55]  
Yang Q(undefined)undefined undefined undefined undefined-undefined
[56]  
Cui C(undefined)undefined undefined undefined undefined-undefined
[57]  
Shen YQ(undefined)undefined undefined undefined undefined-undefined
[58]  
Diaz Heijtz R(undefined)undefined undefined undefined undefined-undefined
[59]  
Wang S(undefined)undefined undefined undefined undefined-undefined
[60]  
Anuar F(undefined)undefined undefined undefined undefined-undefined