Effects of Indocyanine green on cultured retinal ganglion cells in-vitro

被引：10

作者：

Balaiya S. ^{[1
]}

Brar V. ^{[1
]}

Murthy R. ^{[1
]}

Chalam K.V. ^{[1
]}

机构：

[1] University of Florida College of Medicine, Department of Ophthalmology, Jacksonville, FL

来源：

BMC Research Notes | / 2卷 / 1期

关键词：

Retinal Ganglion Cell; Indocyanine Green; Macular Hole; Diabetic Macular Edema; Internal Limit Membrane;

D O I：

10.1186/1756-0500-2-236

中图分类号：

学科分类号：

摘要：

Background. Indocyanine green (ICG) dye is commonly used to stain the inner limiting membrane during macular surgery. There are reports documenting the toxicity of ICG on retinal pigment epithelial cells, with conflicting results in retinal ganglion cells. In the present study, we evaluated the effect of ICG on retinal ganglion cells in vitro. Cultured rat retinal ganglion cells (RGC-5) were exposed to different concentrations of ICG (0.25, 0.5, 1.0, 1.25, & 5 mg/ml) and at various time intervals (1, 5, 15, 30, & 60 minutes). Changes in structural morphology were identified using phase contrast bright field microscopy. Cell viability was quantified using the neutral red assay and cell death was characterized using Annexin-V staining. Findings. Significant morphologic changes were observed at the 15 and 60 min intervals for all concentrations, where a reduction in cell size and loss of normal spindle shape was noted. A dose dependent decrease in cell viability was observed with increasing concentration of ICG as well as increasing exposure intervals. Compared to control, 48-74% reduction in neutral red uptake at all concentrations for exposures 5 min or greater (p < 0.001). Even at 1 min exposure, a dose dependent decline was observed in cell viability, with a 28-48% decline for doses above 1.25 mg/ml (p = 0.007). Staining with Annexin-V, demonstrated a similar dose and time dependent increase in number of cells exhibiting early apoptosis. A greater than two-fold increase in Annexin-V expression for all doses at exposures greater than 1 min was noted. Conclusion. ICG dye exhibits toxicity to retinal ganglion cells at clinically relevant doses following 1 min exposure. © 2009 Chalam et al; licensee BioMed Central Ltd.

引用

共 21 条

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