Effect of the ABRUPTUS/PINOID gene on expression of the LEAFY gene in Arabidopsis thaliana

被引：0
作者：
O. V. Lebedeva
U. N. Ondar
A. A. Penin
T. A. Ezhova
机构：
[1] Moscow State University,Department of Genetics
来源：
Russian Journal of Genetics | 2005年 / 41卷
关键词：
Nucleotide; Protein Kinase; Nucleotide Sequence; Glutamic Acid; Arabidopsis Thaliana;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The nucleotide sequence was analyzed for the temperature-sensitive allele abruptus (abr), which distorts polar auxin transport (PAT) in the inflorescence. The mutation C → T was found in the second exon and led to an amino acid substitution (glycin → glutamic acid) in the conserved domain of protein kinase encoded by the ABRUPTUS/PINOID (ABR/PID) gene. QRT-PCR revealed a 100-fold decrease in transcription of the LEAFY (LFY) gene in the abr mutant with high expressivity of the mutant character; transcription of the fused LFY::GUS gene was also low in the mutant. The results agree with data of the phenotypic analysis of the abr lfy double mutant and testify to an important role of auxin gradients in regulating the expression pattern of the LFY gene.
引用
收藏
页码：445 / 451
页数：6
相关论文
共 99 条
  • [11] Weigel D.(1999) (L.) Heynh. Rus. J. Genet. 35 1312-1325
  • [12] Mandel M.A.(1983)Interaction between the Mol. Biol. Rep. 1 19-21
  • [13] Koornneef M.(1987) and EMBO J. 6 3901-3907
  • [14] Alonso-Blanco C.(1999) Genes during Floral Morphogenesis in Plant J. 18 395-405
  • [15] Peeters A.J.M.(1994) (L.) Haynh. Gene 143 211-216
  • [16] Spooe W.(2000)Role of the Cell 100 469-478
  • [17] Mouradov A.(2001) Gene, Controlling Morphogenesis in Development 128 4057-4067
  • [18] Cremer F.(1998) (L.) Heynh., in Regulating the Growth and Morphogenesis in an in Vitro Culture Science 282 2226-2230
  • [19] Coupland G.(1991) (L.) Heynh. As a Model Object for Studying Genetic Control of Morphogenesis Plant Cell 3 677-684
  • [20] Benett S.R.M.(1993)A Plant DNA Minipreparation: Version II. Plant Plant Cell 5 621-630
12345678910