In vitro hepatoprotective and antioxidant activities of crude extract and isolated compounds from Ficus gnaphalocarpa

被引：12

作者：

Hubert D.J. ^{[1
]}

Dawe A. ^{[2
]}

Florence N.T. ^{[3
]}

Gilbert K.D.W.F. ^{[4
]}

Angele T.N. ^{[1
]}

Buonocore D. ^{[5
]}

Finzi P.V. ^{[6
]}

Vidari G. ^{[6
]}

Bonaventure N.T. ^{[2
]}

Marzatico F. ^{[5
]}

Paul M.F. ^{[1
]}

机构：

[1] Department of Biochemistry, Faculty of Science, University of Yaounde i, Yaoundé

[2] Department of Organic Chemistry, Faculty of Science, University of Yaounde i, Yaoundé

[3] Department of Animal Biology and Physiology, Faculty of Science, University of Yaounde i, Yaoundé

[4] Department of Chemistry, Higher Teachers' Training College, University of Yaounde i, Yaoundé

[5] Dipartimento di Medicina Legale, Sc. Forensi e Farmacotossicologiche Sezione di Sc. Farmacologiche e Tossicologiche, Università Degli Studi di Pavia, Pavia 27100

[6] Dipartimento di Chimica Organica, Centre of Ethnobiopharmacy (CISTRE), Università Degli Studi di Pavia, Pavia 27100

来源：

Inflammopharmacology | 2011年 / 19卷 / 1期

关键词：

Antioxidant activity; Carbon tetrachloride; Ficus gnaphalocarpa; Free radicals; Hepatoprotective effect; Plant compounds; Silymarin;

D O I：

10.1007/s10787-010-0070-4

中图分类号：

学科分类号：

摘要：

The in vitro hepatoprotective effect of the methanolic extract from Ficus gnaphalocarpa (Miq.) Steud. ex A. Rich (Moraceae) on the CCl4-induced liver cell damage as well as the possible antioxidant mechanisms involved in this protective effect, were investigated. The phytochemical investigation of this methanolic extract led to the isolation of six compounds identified as: betulinic acid (1); 3-methoxyquercetin (2); catechin (3); epicatechin (4); quercetin (5); and quercitrin (6). The hepatoprotective activity of these compounds was tested in vitro against CCl4-induced damage in rat hepatoma cells. In addition, radical-scavenging activity, β-carotene- linoleic acid model system, ferric-reducing antioxidant parameter and microsomal lipid peroxidation assays were used to measure antioxidant activity of crude extract and isolated compounds. Silymarin and trolox were used as standard references and, respectively, exhibited significant hepatoprotective and antioxidant activities. (5), (6) and (2) showed significant antioxidant and hepatoprotective activities as indicated by their ability to prevent liver cell death and lactate dehydrogenase leakage during CCl4 intoxication. These results suggest that the protective effects of crude extract of F. gnaphalocarpa against the CCl4-induced hepatotoxicity possibly involve the antioxidant effect of these compounds. © 2010 Springer Basel AG.

引用

页码：35 / 43

页数：8

共 43 条

[31] Naseem N.Q., Bhanudansh S.K., Nadeem A.L., Et al., Antioxidant and hepatoprotective activity of Cordia macleodii leaves, Saudi Pharm J, 17, pp. 299-302, (2009)
[32] Negre-Salvayre A., Salvayre R., Quercetin prevents the cytotoxicity of oxidized LDL on lymphoid cell lines, Free Radic Biol Med, 12, pp. 101-106, (1992)
[33] Ning W., Peibo L., Yonggang W., Et al., Hepatoprotective effect of Hypericum japonicum extract and its fractions, J Ethnopharmacol, 116, pp. 1-6, (2008)
[34] Nongonierma R.B., Ndiaye L., Thiam D., Et al., Activité antidrépanocytaire de la fraction F3 de l'extrait acétonique des écorces des tiges de Ficus gnaphalocarpa (Mig) steud (Moraceae), Pharmacien d'Afrique, 187, pp. 3-6, (2005)
[35] Rajesh K., Ramasamy M., Ramtej J., Et al., Isolation, characterization and antioxidative effect of phyllanthin against CCl<sub>4</sub>-induced toxicity in HepG2 cell line, Chem Biol Interact, 181, pp. 351-358, (2009)
[36] Rodeiro I., Donato M.T., Martinez I., Et al., Potential hepatoprotective effects of new Cuban natural products in rat hepatocytes culture, Toxicol in Vitro, 22, pp. 1242-1249, (2008)
[37] Rubinstein D., Epinephrine release and liver glycogen levels after carbon tetrachloride administration, Am J Physiol, 203, pp. 1033-1037, (1962)
[38] Saad B., Abu-Hijleh G., Suter U.W., Polymer biocompatibility assessment by cell culture techniques, The PMB Series: Introduction Polymeric Biomaterials, 1, (2003)
[39] Sesso H.D., Gaziano J.M., Liu S., Buring J.E., Flavonoid intake and the risk of cardiovascular disease in women, American Journal of Clinical Nutrition, 77, 6, pp. 1400-1408, (2003)
[40] Suresh R.N., Vandana S.P., Hepatoprotective effect of Ginkgoselect Phytosome<sup>®</sup> in rifampicin induced liver injury in rats: Evidence of antioxidant activity, Fitoterapia, 79, pp. 439-445, (2008)

← 1 2 3 4 5 →