Time-resolved methods in biophysics. 7. Photon counting vs. analog time-resolved singlet oxygen phosphorescence detection

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作者
Ana Jiménez-Banzo
Xavier Ragàs
Peter Kapusta
Santi Nonell
机构
[1] Institut Químic de Sarrià,Grup d’Enginyeria Molecular
[2] Universitat Ramon Llull,undefined
[3] Via Augusta 390,undefined
[4] PicoQuant GmbH,undefined
[5] Rudower Chaussee 29,undefined
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摘要
Two recent advances in optoelectronics, namely novel near-IR sensitive photomultipliers and inexpensive yet powerful diode-pumped solid-state lasers working at kHz repetition rate, enable the time-resolved detection of singlet oxygen (O2(a1Δg)) phosphorescence in photon counting mode, thereby boosting the time-resolution, sensitivity, and dynamic range of this well-established detection technique. Principles underlying this novel approach and selected examples of applications are provided in this perspective, which illustrate the advantages over the conventional analog detection mode.
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页码:1003 / 1010
页数:7
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