Molecular cloning, sequencing, and expression analysis of presenilin cDNA from Schistosoma mansoni

被引：0
作者：
Lizandra Guidi Magalhães
William de Castro-Borges
Matheus de Souza Gomes
Renata Guerra-Sá
Vanderlei Rodrigues
机构：
[1] Universidade de São Paulo,Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto
[2] Universidade Federal de Ouro Preto,Departamento de Ciências Biológicas, Instituto de Ciências Exatas e Biológicas
来源：
Parasitology Research | 2009年 / 106卷
关键词：
Schistosoma Mansoni; Schistosoma Japonicum; Aspartyl Protease; Transcriptome Database; NCBInr Database;
D O I：
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中图分类号：
学科分类号：
摘要：
Presenilins (PS) are integral membrane proteins involved, among other functions, in regulated intramembrane proteolysis. In this study, we report the identification and characterization of a complementary DNA from Schistosoma mansoni exhibiting a significant homology to human and nonvertebrate presinilins. S. mansoni contained a 1,485 bp open reading frame encoding a predicted protein of 494 amino acids. Alignment of predicted amino acid sequence of S. mansoni with PS (SmPS) from other species revealed up to 40% similarity shared among the investigated organisms. In addition, phylogenetic analyses demonstrated SmPS being closely related to its orthologues found in Schistosoma japonicum and Caenorhabditis elegans. Expression analysis of SmPS using quantitative real-time PCR revealed that the transcript is up-regulated in the egg stage. We hypothesize that the high level of SmPS in the S. mansoni embryo correlates to an important role during cellular signaling associated to larval development. To our knowledge, this study represents the first attempt to investigate the existence and abundance of PS from a helminth parasite.
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页码：7 / 13
页数：6
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