In vivo dynamics of human cord blood-derived CD34− SCID-repopulating cells using intra-bone marrow injection

被引:0
作者
T Kimura
Y Matsuoka
M Murakami
T Kimura
M Takahashi
T Nakamoto
K Yasuda
K Matsui
K Kobayashi
S Imai
H Asano
R Nakatsuka
Y Uemura
Y Sasaki
Y Sonoda
机构
[1] Graduate School of Medical Science,Department of Stem Cell Biology and Regenerative Medicine
[2] Kansai Medical University,First Department of Internal Medicine
[3] Moriguchi,Department of Pediatrics
[4] Kansai Medical University,Department of Obstetrics and Gynecology
[5] Moriguchi,Department of Gynecology and Obstetrics
[6] Kansai Medical University,Department of Obstetrics and Gynecology
[7] Moriguchi,undefined
[8] Kansai Medical University,undefined
[9] Moriguchi,undefined
[10] Fukuda Hospital,undefined
[11] Kumamoto,undefined
[12] Aizenbashi Hospital,undefined
[13] School of Nursing,undefined
[14] Kyoto Prefectural University of Medicine,undefined
来源
Leukemia | 2010年 / 24卷
关键词
SCID-repopulating cell; IBMI; CD34; CD38; hematopoietic stem cell; cord blood;
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中图分类号
学科分类号
摘要
The identification of human CD34-negative (CD34−) hematopoietic stem cells (HSCs) provides a new concept for the hierarchy in the human HSC compartment. This study investigated the long-term repopulating capacity and redistribution kinetics of human cord blood-derived CD34− severe combined immunodeficiency (SCID)-repopulating cells (SRCs) and compared them with those of CD34+CD38+ and CD34+CD38− SRCs using the intra-bone marrow injection (IBMI) to clarify the characteristics of CD34− SRCs. On the basis of the limiting dilution analyses data, estimated numbers of CD34+CD38+, CD34+CD38−, and CD34− SRCs were transplanted to NOD/SCID mice by IBMI. The human cell repopulation at the site of injection and the other bones were serially investigated. Interestingly, CD34+CD38+, CD34+CD38−, and CD34− SRCs began to migrate to other bones 2 and 5 weeks after the transplantation, respectively. Accordingly, the initiation of migration seemed to differ between the CD34+ and CD34− SRCs. In addition, CD34+CD38+ SRCs only sustained a short-term repopulation. However, both CD34+CD38− and CD34− SRCs had longer-term repopulation capacity. Taken together, these findings showed that CD34− SRCs show different in vivo kinetics, thus suggesting that the identified CD34− SRCs are a distinct class of primitive HSCs in comparison to the CD34+CD38+ and CD34+CD38− SRCs.
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页码:162 / 168
页数:6
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