Effect of extra- and intra-mitochondrial calcium on citrulline synthesis

被引：0
作者：
A. Saavedra-Molina
T. M. Devlin
机构：
[1] Universidad Michoacana de San Nicolás de Hidalgo,Instituto de Investigaciones Químico
[2] Hahnemann University School of Medicine,Biológicas
来源：
Amino Acids | 1997年 / 12卷
关键词：
Amino acids; Liver; Mitochondria; Calcium; Citrulline; Ruthenium red;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A quantitative relationship between rat liver mitochondrial matrix free Ca2+ ([Ca2+]m) and citrullinogenesis has been observed. Maximum citrulline synthesis occurred at 100 to 200nM [Ca2+]m; higher [Ca2+]m caused inhibition. When [Ca2+]m was decreased to below 50nM, by addition of A23187 and EGTA, inhibition also occurred. By incubating mitochondria with ruthenium red ([Ca2+]m = 200nM) prior to addition of extramitochondrial free Ca2+ ([Ca2+]o) it was found that high external Ca2+ (800nM) did not inhibit citrulline synthesis thus demonstrating that [Ca2+]m, not [Ca2+]o was controlling citrullinogenesis.
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页码：293 / 298
页数：5
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