CRISPR–Cas12b enables efficient plant genome engineering

被引：0

作者：

Meiling Ming

Qiurong Ren

Changtian Pan

Yao He

Yingxiao Zhang

Shishi Liu

Zhaohui Zhong

Jiaheng Wang

Aimee A. Malzahn

Jun Wu

Xuelian Zheng

Yong Zhang

Yiping Qi

机构：

[1] University of Maryland,Department of Plant Science and Landscape Architecture

[2] Nanjing Agricultural University,Centre of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement

[3] University of Electronic Science and Technology of China,Department of Biotechnology, School of Life Science and Technology, Center for Informational Biology

[4] University of Maryland,Institute for Bioscience and Biotechnology Research

来源：

Nature Plants | 2020年 / 6卷

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摘要：

Clustered regularly interspaced short palindromic repeats (CRISPR)–Cas12b is a newly emerged genome engineering system. Here, we compared Cas12b from Alicyclobacillus acidoterrestris (Aac), Alicyclobacillus acidiphilus (Aa), Bacillus thermoamylovorans (Bth) and Bacillus hisashii (Bh) for genome engineering in rice, an important crop. We found AaCas12b was more efficient than AacCas12b and BthCas12b for targeted mutagenesis, which was further demonstrated in multiplexed genome editing. We also engineered the Cas12b systems for targeted transcriptional repression and activation. Our work establishes Cas12b as the third promising CRISPR system, after Cas9 and Cas12a, for plant genome engineering.

引用

页码：202 / 208

页数：6