Optimization of medium constituents and fermentation conditions for the production of L-Glutamic acid by the coimmobilized whole cells of Micrococcus glutamicus and Pseudomonas reptilivora

被引：0

作者：

I. Sunitha

M. V. Subba Rao

C. Ayyanna

机构：

[1] Biotechnology laboratories,

[2] Department of Chemical Engineering,undefined

[3] College of Engineering,undefined

[4] Andhra University,undefined

[5] Visakhapatnam 530 003,undefined

[6] India,undefined

[7] Department of Environmental Sciences,undefined

[8] College of Science and Technology,undefined

[9] Andhra University,undefined

[10] Visakhapatnam 530 003,undefined

[11] India,undefined

来源：

Bioprocess Engineering | 1998年 / 18卷

关键词：

Glucose; Fermentation; Urea; Pseudomonas; Biotin;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Statistical experimental design was used to optimize medium constituents and the conditions of fermentation, viz., temperature, pH and the time of fermentation. Higher yields of L-glutamic acid (37.1 kg/m3) was obtained after optimizing medium components and the conditions of fermentation. The optimal levels of medium components were: 61.5575 kg/m3 glucose, 7.3272 kg/m3 urea and 1.783 μg/dm3 biotin. The optimum productivity was achieved using optimized medium at the fermentation temperature of 33.7 °C, initial pH 7.74, and at the time of fermentation of 58.4 h.

引用

页码：353 / 359

页数：6