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Coexpression of the Bacillus pumilusβ-xylosidase (xynB) gene with the Trichoderma reeseiβ-xylanase 2 (xyn2) gene in the yeast Saccharomyces cerevisiae
被引：0
作者：
D. C. La Grange
M. Claeyssens
I. S. Pretorius
W. H. Van Zyl
机构：
[1] Department of Microbiology,
[2] University of Stellenbosch,undefined
[3] Victoria Street,undefined
[4] Stellenbosch 7600,undefined
[5] South Africa e-mail: whvz@maties.sun.ac.za Tel.: +27-21-8085854 Fax: +27-21-8085846,undefined
[6] Institute for Wine Biotechnology,undefined
[7] University of Stellenbosch,undefined
[8] Victoria Street,undefined
[9] Stellenbosch 7600,undefined
[10] South Africa,undefined
[11] Department of Biochemistry,undefined
[12] Physiology and Microbiology,undefined
[13] University of Ghent,undefined
[14] Ghent 9000,undefined
[15] Belgium,undefined
来源：
Applied Microbiology and Biotechnology
|
2000年
/
54卷
关键词：
Fusion Protein;
Saccharomyces Cerevisiae;
Trichoderma;
Alcohol Dehydrogenase;
Transcriptional Control;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The xynB gene encoding the Bacillus pumilusβ-xylosidase was expressed separately and jointly with the Trichoderma reeseiβ-xylanase (xyn2) gene in the yeast Saccharomyces cerevisiae. Both genes were placed under the transcriptional control of the glucose-derepressible alcohol dehydrogenase 2 promoter (ADH2P) and terminator (ADH2T) sequences. The xynB gene was fused in frame to the yeast mating factor α1 secretion sequence (MFα1S) to effect secretion in S. cerevisiae. The fusion protein was designated Xlo1. Xlo1 produced in S. cerevisiae exhibited low affinity for xylobiose, but eventually hydrolyzed xylobiose and xylotriose to the monomeric constituent, d-xylose. Coproduction of Xyn2 and Xlo1 by S. cerevisiae led to a 25% increase in the amount of reducing sugars released from birchwood xylan compared to S. cerevisiae producing only the Xyn2 β-xylanase. However, no d-xylose was produced from birchwood xylan, presumably due to very low Xlo1 β-xylosidase activity and its low affinity for xylobiose.
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页码：195 / 200
页数：5
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