A microscale anisotropic biaxial cell stretching device for applications in mechanobiology

被引：0
作者：
Dominique Tremblay
Sophie Chagnon-Lessard
Maryam Mirzaei
Andrew E. Pelling
Michel Godin
机构：
[1] University of Ottawa,Department of Physics
[2] University of Ottawa,Ottawa
[3] University of Ottawa,Carleton Institute for Biomedical Engineering
[4] University of Ottawa,Department of Biology
来源：
Biotechnology Letters | 2014年 / 36卷
关键词：
Anisotropic deformation; Cell stretching; Live-cell imaging; Mechanobiology; Microfabrication; Microfluidics;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A multi-layered polydimethylsiloxane microfluidic device with an integrated suspended membrane has been fabricated that allows dynamic and multi-axial mechanical deformation and simultaneous live-cell microscopy imaging. The transparent membrane’s strain field can be controlled independently along two orthogonal directions. Human foreskin fibroblasts were immobilized on the membrane’s surface and stretched along two orthogonal directions sequentially while performing live-cell imaging. Cyclic deformation of the cells induced a reversible reorientation perpendicular to the direction of the applied strain. Cells remained viable in the microdevice for several days. As opposed to existing microfluidic or macroscale stretching devices, this device can impose changing, anisotropic and time-varying strain fields in order to more closely mimic the complexities of strains occurring in vivo.
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页码：657 / 665
页数：8
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