Detection of antibodies against viral capsid proteins of human herpesvirus 8 in AIDS-associated Kaposi’s sarcoma

被引：0

作者：

Stefanie André

Octavian Schatz

Johannes R. Bogner

Heinz Zeichhardt

Marina Stöffler-Meilicke

Hans-Ulrich Jahn

Reiner Ullrich

Anne-Katharina Sonntag

Roland Kehm

J. Haas

机构：

[1] Max-von-Pettenkofer Institut für Virologie,

[2] Genzentrum,undefined

[3] Universität München,undefined

[4] Feodor-Lynen-Strasse 25,undefined

[5] D-81377 Munich,undefined

[6] Germany,undefined

[7] Medizinische Poliklinik,undefined

[8] Universität München,undefined

[9] Pettenkofer Strasse 8a,undefined

[10] D-80336 Munich,undefined

[11] Germany,undefined

[12] Institut für Klinische und Experimentelle Virologie,undefined

[13] Universitätsklinikum Benjamin Franklin,undefined

[14] Freie Universität Berlin,undefined

[15] Hindenburgdamm 27,undefined

[16] D-12203 Berlin,undefined

[17] Germany,undefined

[18] Medizinische Klinik,undefined

[19] Universitätsklinikum Benjamin Franklin,undefined

[20] Freie Universität Berlin,undefined

[21] Hindenburgdamm 30,undefined

[22] D-12200 Berlin,undefined

[23] Germany,undefined

[24] Universitätshautklinik,undefined

[25] Universität Heidelberg,undefined

[26] Voßstrasse 2,undefined

[27] D-69115 Heidelberg,undefined

[28] Germany,undefined

[29] Institut für Medizinische Virologie,undefined

[30] Universität Heidelberg,undefined

[31] Im Neuenheimer 324,undefined

[32] D-69120 Heidelberg,undefined

[33] Germany,undefined

来源：

Journal of Molecular Medicine | 1997年 / 75卷

关键词：

Key words Antibody; Capsid antigens; HHV-8; Kaposi’s sarcoma;

D O I：

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中图分类号：

学科分类号：

摘要：

Sequences of a new herpesvirus with homology to gammaherpesvirinae were recently identified in AIDS-associated Kaposi’s sarcoma (KS). Subsequently this novel virus, called KS-associated virus (KSHV) or human herpesvirus (HHV) 8 was detected in classical KS and AIDS-associated body cavity based lymphomas by polymerase chain reaction. In this report major and minor capsid proteins of HHV-8 were molecularly cloned and produced as recombinant proteins in Escherichia coli. Sera from 69 HIV-1 infected patients with KS, 30 HIV-1 infected patients without KS and 106 control individuals were tested by enzyme-linked immunosorbent assay for anti-HHV-8 capsid IgM and IgG antibodies. Sera from four patients were tested over periods ranging from 18 months to 6 years. IgG antibodies directed against HHV-8 capsid antigens were detected in patients with AIDS-associated KS and in some AIDS patients without KS. Seroconversion with IgM and IgG antibodies directed against HHV-8 capsid proteins occurred more than 1 year prior to diagnosis of KS. In a considerable portion of KS patients no IgM or IgG antibodies against HHV-8 capsid proteins were detected. In these patients there was an inverse relationship between antibodies against HHV-8orf26 and the CD4/CD8 ratio, suggesting that the inconsistency of anti-HHV-8orf26 antibodies is due at least partly to an impaired immune response. No reactivity against HHV-8 capsid antigens was detected in the vast majority of sera from HIV-negative control individuals. Our findings indicate that a specific humoral immune response against capsid proteins is raised in HHV-8 infected individuals, and that anti-capsid antibodies can be used to diagnose HHV-8 infection. The correlation between occurrence of anti-HHV-8 antibodies and KS supports the hypothesis of a causative role of HHV-8.

引用

页码：145 / 152

页数：7