Effects of low-intensity ultrasound on cell proliferation and reproductivity

被引：7

作者：

Yang C. ^{[1
]}

Jiang X. ^{[1
]}

Du K. ^{[1
]}

Cai Q. ^{[2
]}

机构：

[1] Tianjin Key Laboratory of Biomedical Detecting Techniques and Instruments, School of Precision Instrument and Opto-Electronics Engineering, Tianjin University, Tianjin

[2] Department of Urology, Tianjin Institute of Urology, The Second Hospital of Tianjin Medical University, Tianjin

来源：

Transactions of Tianjin University | 2016年 / 22卷 / 02期

关键词：

cell morphology; cell proliferation; cell reproductivity; cell viability; low-intensity ultrasound;

D O I：

10.1007/s12209-016-2614-1

中图分类号：

学科分类号：

摘要：

Ultrasound has been widely used in clinics. Cellular responses to low-intensity ultrasound are parameter-dependent. Proper parameter setting is vital to its exact use. To get guidelines for parameter setting, lowintensity ultrasound stimulation on the proliferation and reproductivity of HepG2 and 3T3 cells in vitro was examined with a 1.06 MHz-generator by changing the parameters(including intensity, pulse repetition frequency and duty cycle)in a wide range. Cell viability and reproductivity at different time after sonication were measured by 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)and colony formation assay to indicate timerelated proliferation. The results illustrate that ultrasound irradiation at 0.4–0.8 W/cm2 and high pulse repetition frequency(100 Hz)can facilitate cell proliferation, while above 0.8 W/cm2 would resist it. The extent of resistance closely correlated with duty cycle and pulse repetition frequency. Resistance effect at low pulse repetition frequency(1 Hz)is greater than that at high pulse repetition frequency(100 Hz)and not time-related. The influence of high pulse repetition frequency is time-accumulated, indicating cellular process involved. These findings would provide valuable guidelines for the application of low-intensity ultrasound in stem cell transformation and tissue engineering. © 2016, Tianjin University and Springer-Verlag Berlin Heidelberg.

引用

页码：125 / 131

页数：6

共 27 条

[1]

Kaminski G.A., Computational studies of the X-linked inhibitor of apoptosis complex formation with caspase-9 and a small antagonist [J], J Chem Theory Comput, 4, 5, pp. 847-854, (2008)

[2]

Juffermans L.J.M., van Dijk A., Jongenelen C.A.M., Et al., Ultrasound and microbubble-induced intra and intercellular bioeffects in primary endothelial cells [J], Ultrasound in Medicine and Biology, 35, 11, pp. 1917-1927, (2009)

[3]

Heckman J.D., Ryaby J.P., McCabe J., Et al., Acceleration of tibial fracture-healing by non-invasive, low intensity pulsed ultrasound [J], J Bone Joint Surg, 76, 1, pp. 26-34, (1994)

[4]

Kristiansen T.K., Ryaby J.P., McCabe J., Et al., Accelerated healing of distal radial fractures with the use of specific, low-intensity ultrasound: A multicenter, prospective, randomized, double-blind, placebo-controlled study [J], J Bone Joint Surg, 79, 7, pp. 961-973, (1997)

[5]

Olkkua A., Leskinen J.J., Lammi M.J., Et al., Ultrasoundinduced activation of Wnt signaling in human MG-63 osteoblastic cells [J], Bone, 47, 2, pp. 320-330, (2010)

[6]

Rutten S., Nolte P.A., Korstjens C.M., Et al., Low-intensity pulsed ultrasound increases bone volume, osteoid thickness and mineral apposition rate in the area of fracture healing in patients with a delayed union of the osteotomized fibula [J], Bone, 43, 2, pp. 348-354, (2008)

[7]

Uglow M.G., Peat R.A., Hile M.S., Et al., Low-intensity ultrasound stimulation in distraction osteogenesis in rabbits [J], Clin Orthop Relat Res, 417, pp. 303-312, (2003)

[8]

Mukai S., Ito H., Nakagawa Y., Et al., Transforming growth factor-beta(1)mediates the effects of low-intensity pulsed ultrasound in chondrocytes [J], Ultrasound Med Biol, 31, 12, pp. 1713-1721, (2005)

[9]

Zhang Z.J., Huckle J., Francomano C.A., Et al., The influence of pulsed low-intensity ultrasound on matrix production of chondrocytes at different stages of differentiation: An explants study [J], Ultrasound Med Biol, 28, pp. 1547-1553, (2002)

[10]

Kopakkala-Tani M., Leskinen J.J., Karjalainen H.M., Et al., Ultrasound stimulates proteoglycan synthesis in bovine primary chondrocytes [J], Biorheology, 43, 3-4, pp. 271-282, (2006)

← 1 2 3 →