As one of the three major chronic diseases, diabetes often causes many complications, which can affect various parts of the body and even threaten the life of the patients. At present, the situation of diabetes in the world is quite serious. Accurate detection of blood glucose is very important for the diagnosis, treatment and medication of diabetes as well as the self-management of diabetic patients. In this paper, an electrochemical glucose biosensor was developed based on screen-printed electrode (SPE) modified with composite material of graphene aerogel (GA) and Prussian blue (PB) (denoted as GA@PB), which was fabricated via chemical reduction using L-ascorbic acid as a reducing agent through a freeze-drying process. Glucose was specifically captured by glucose oxidase (GOx) which were immobilized into the GA@PB by chitosan. The structure and performance of the sensor were characterized by scanning electron microscopy (SEM), Raman spectroscopy measurements, Fourier transform infrared spectrometer (FTIR), cyclic voltammetry (CV) and amperometric detection. The sensor exhibited a linear range of 0.5–6.0 mmol·L−1 with limit of detection (LOD) of 0.15 mmol·L−1, indicating that the combination of graphene aerogel and Prussian blue possess well conductivity and catalytic performance.
