Detection of Citrus psorosis virus Using an Improved One-Step RT-PCR

被引：0

作者：

Asmae Achachi

Mohamed Haïssam Jijakli

Elmostafa El Fahime

Abdelmajid Soulaymani

Mohammed Ibriz

机构：

[1] Université Ibn Tofaïl,Laboratoire de Génétique et Biométrie, Département de Biologie, Faculté des Sciences

[2] Gembloux Agro-Bio Tech,Unité de Phytopathologie

[3] Université de Liège,Plateforme Génomique Fonctionnelle, UATRS

[4] Centre National Pour la Recherche Scientifique et Technique (CNRST),undefined

来源：

Arabian Journal for Science and Engineering | 2015年 / 40卷

关键词：

Psorosis; CPsV detection; Biological indexing; ELISA; One-step RT-PCR;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

We report an improvement of a one-step reverse transcription polymerase chain reaction (RT-PCR) assay for the detection of Citrus psorosis virus (CPsV; genus Ophiovirus) in citrus trees. Two different sample preparation procedures were compared. The data showed that when using total RNA extracted by the Qiagen procedure more virus isolates could be detected compared with the Trizol procedure. Three pairs of primers reported in literature and selected within the coat protein (CP) region of the virus were used for a reliable detection of CPsV. Only one primer pair has been able to detect all CPsV isolates collected from different citrus regions of Morocco. In addition, this report showed that one-step RT-PCR was more sensitive and more consistent than ELISA test. Notably, 15 out of 22 samples tested positive by one-step RT-PCR could not be detected by Mab Ps29, thus providing a degree of differentiation among isolates. The detection protocol described in this study could be used in citrus certification programs and to test trees in nurseries and commercial orchards for CPsV infection.

引用

页码：7 / 13

页数：6

共 68 条

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