Preparation and evaluation of oleoyl-carboxymethy-chitosan (OCMCS) nanoparticles as oral protein carriers

被引：0
作者：
Ya Liu
Xiao Jie Cheng
Qi Feng Dang
Fang Kui Ma
Xi Guang Chen
Hyun Jin Park
Bum Keun Kim
机构：
[1] Ocean University of China,College of Marine Life Science
[2] Korea University,College of Life Sciences and Biotechnology
[3] Korea Food Research Institute,undefined
来源：
Journal of Materials Science: Materials in Medicine | 2012年 / 23卷
关键词：
Chitosan; Zeta Potential; Protein Drug; Aeromonas Hydrophila; Mucoadhesive Property;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Oleoyl-carboxymethy chitosan (OCMCS) nanoparticles based on chitosan with different molecular weights (50, 170 and 820 kDa) were prepared by self-assembled method. The nanoparticles had spherical shape, positive surface charges and the mean diameters were 157.4, 274.1 and 396.7 nm, respectively. FITC-labeled OCMCS nanoparticles were internalized via the intestinal mucosa and observed in liver, spleen, intestine and heart following oral deliverance to carps (Cyprinus carpio). Extracellular products (ECPs) of Aeromonas hydrophila as microbial antigen was efficiently loaded to form OCMCS–ECPs nanoparticles and shown to be sustained release in PBS. Significantly higher (P < 0.05) antigen-specific antibodies were detected in serum after orally immunized with OCMCS-ECPs nanoparticles than that immunized with ECPs alone and non-immunized in control group in carps. These results implied that amphiphilic modified chitosan nanoparticles had great potential to be applied as carriers for the oral administration of protein drugs.
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页码：375 / 384
页数：9
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