Multichannel Confocal Microscope Based on a Diffraction Focusing Multiplier with Automatic Synchronization of Scanning

被引:2
作者
Bessmeltsev V.P. [1 ]
Maksimov M.V. [1 ]
Vileiko V.V. [1 ]
Goloshevskii N.V. [1 ]
Terent’ev V.S. [1 ]
机构
[1] Institute of Automation and Electrometry, Siberian Branch, Russian Academy of Sciences, pr. Akademika Koptyuga 1, Novosibirsk
关键词
diffractive optical element; multichannel confocal microscope; shifted plane-parallel plates;
D O I
10.3103/S8756699018060018
中图分类号
学科分类号
摘要
Implementation of a laser scanning confocal microscope is described, where the specimen is scanned by an array of illuminating beams, which significantly increases the velocity of object image construction. The array formation is provided by using a diffractive optical element. Scanning by the array of laser beams over the specimen is performed by galvanometric scanners with moving refractive plane-parallel plates. Owing to application of such a scanning device, the beams in the illuminating channel and the signal beams in the receiving channel pass through one motionless array of confocal diaphragms; as a result, the scanning beams in the specimen plane and the signal beams in the plane of the photodetector matrix can be used without an additional synchronized pair of scanners. The proposed confocal microscope can be applied in problems that require a fast response. © 2018, Allerton Press, Inc.
引用
收藏
页码:531 / 537
页数:6
相关论文
共 15 条
[1]  
Pawley J.B., Handbook of Biological Confocal Microscopy, (2006)
[2]  
Svishchev G.M., Confocal Microscopy and Ultramicroscopy of a Living Cell, (2011)
[3]  
NS-3000 High-Speed Laser Confocal 3D Microscope
[4]  
Ishihara M., Sasaki H., High-Speed Surface Measurement Using a Non-Scanning Multiple-Beam Confocal Microscope, Opt. Eng., 38, 6, pp. 1035-1040, (1999)
[5]  
Bessmeltsev V.P., Zavyalov P.S., Korolkov V.P., Et al., Diffractive Focusing Fan-Out Element for the Parallel DNA Sequencer, Avtometriya, 53, 5, pp. 48-56, (2017)
[6]  
Krantz M.C., Conventional and Confocal Multi-Spot Scanning Optical Microscope
[7]  
Karin J., Golub M., Confocal Microscope with Diffractively Formed Virtual Pinhole Array, (2004)
[8]  
Shimozawa T., Yamagata K., Kondo T., Et al., Improving Spinning Disk Confocal Microscopy by Preventing Pinhole Cross-Talk for Intravital Imaging, Proc. Natl. Acad. Sci. USA., 110, 9, pp. 3399-3404, (2013)
[9]  
Bessmel'tsev V.P., Terent'ev V.S., Calculating the Spatial Fluorescence Distribution of a Thick Fluorophore Layer in a Mutlichannel Confocal Microscope, Opt. Zh., 82, 6, pp. 58-65, (2015)
[10]  
Tanaami T., Otsuki S., Tomosada N., Et al., High-Speed 1-Frame/ms Scanning Confocal Microscope with a Microlens and Nipkow Disks, Appl. Opt., 41, 22, pp. 4704-4708, (2002)