CCR7 pathway induces epithelial–mesenchymal transition through up-regulation of Snail signaling in gastric cancer

被引：8
作者：
Jianping Zhang
Yunzhe Zhou
Yonggang Yang
机构：
[1] The First Hospital of Shijiazhuang City,The Third Department of Geriatrics
[2] The First Hospital of Shijiazhuang City,The Third Department of Oncology
来源：
Medical Oncology | 2015年 / 32卷
关键词：
CCR7; Snail; EMT; Gastric cancer;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The chemokine receptor 7 (CCR7) and Snail signaling have been linked to various types of cancers. The associations between these signalings and the epithelial–mesenchymal transition (EMT) are not clear in gastric cancer. Here, the expression of CCR7 and Snail was detected in gastric cancer by immunohistochemistry and Western blot. Meanwhile, gastric cancer cells were subjected to CCL19, si-control, and si-Snail treatment. Cell cycle, migration, and invasion were also analyzed. The expression patterns of CCR7 and Snail were similar in either gastric cancer tissues or cells. The increased expression of CCR7 was closely associated with the increased Snail expression, which both were closely correlated with metastasis, stage and differentiation, and poor prognosis. The increased p-ERK, p-AKT, Snail, and MMP9 expression and the decreased E-cadherin were confirmed in MGC803 cells in a dose-dependent manner in response to CCL19 treatment. However, the blockade of Snail abrogated the up-regulation of MMP9 and down-regulation of E-cadherin. CCR7-induced ERK and PI3K pathway regulated Snail signaling. Besides si-Snail treatment led to MGC803 cell cycle arrest and affected the migration and invasion. In conclusion, our study suggested that CCR7 promotes Snail expression to induce the EMT, resulting in cell cycle progression, migration, and invasion in gastric cancer. CCR7–Snail pathway provided more potential regimens for cancer therapy.
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