Histone H3K27 acetylation is dispensable for enhancer activity in mouse embryonic stem cells

被引：0

作者：

Tiantian Zhang

Zhuqiang Zhang

Qiang Dong

Jun Xiong

Bing Zhu

机构：

[1] Chinese Academy of Sciences,National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics

[2] University of Chinese Academy of Sciences,College of Life Sciences

来源：

Genome Biology | / 21卷

关键词：

H3K27 acetylation; Enhancer; H3.3; Gene transcription;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

H3K27ac is well recognized as a marker for active enhancers and a great indicator of enhancer activity. However, its functional impact on transcription has not been characterized. By substituting lysine 27 in histone variant H3.3 with arginine in mouse embryonic stem cells, we diminish the vast majority of H3K27ac at enhancers. However, the transcriptome is largely undisturbed in these mutant cells, likely because the other enhancer features remain largely unchanged, including chromatin accessibility, H3K4me1, and histone acetylation at other lysine residues. Our results clearly reveal that H3K27ac alone is not capable of functionally determining enhancer activity.

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