Potential for artefacts in the measurement of fructose and sucrose in extracts of potato tubers using the microplate reader assay

被引:0
作者
S. Mitchell
P. Dobson
H. V. Davies
R. Viola
机构
[1] Scottish Crop Research Institute,
来源
Potato Research | 1998年 / 41卷
关键词
alcohol dehydrogenase; phosphoglucose isomerase; sugar assay; L.;
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摘要
Artefactual results were obtained during assay of sugars in ethanolic extracts of potato tubers with the microplate method. The problem was attributed to contamination with alcohol dehydrogenase of the commercial (yeast) phosphoglucose isomerase used in the assay. The use of phosphoglucose isomerase from another source (rabbit muscle) eliminated the problem.
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页码:383 / 386
页数:3
相关论文
共 2 条
  • [1] Viola R.(1992)A microplate reader assay for rapid enzymatic quantification of sugars in potato tubers Potato Research 35 55-58
  • [2] Davies H.V.(undefined)undefined undefined undefined undefined-undefined