Alternatives to in vivo Draize rabbit eye and skin irritation tests with a focus on 3D reconstructed human cornea-like epithelium and epidermis models

被引：74
作者：
Lee M. [1 ]
Hwang J.-H. [1 ]
Lim K.-M. [1 ]
机构：
[1] College of Pharmacy, Ewha Womans University, Seoul
来源：
Toxicological Research | 2017年 / 33卷 / 3期
关键词：
3D reconstructed human cornea-like epithelium (RhCE) models; 3D reconstructed human epithelium (RhE) models; Alternative to animal tests; Eye irritation; Skin irritation;
D O I：
10.5487/TR.2017.33.3.191
中图分类号：
学科分类号：
摘要：
Human eyes and skin are frequently exposed to chemicals accidentally or on purpose due to their external location. Therefore, chemicals are required to undergo the evaluation of the ocular and dermal irritancy for their safe handling and use before release into the market. Draize rabbit eye and skin irritation test developed in 1944, has been a gold standard test which was enlisted as OECD TG 404 and OECD TG 405 but it has been criticized with respect to animal welfare due to invasive and cruel procedure. To replace it, diverse alternatives have been developed: (i) For Draize eye irritation test, organotypic assay, in vitro cytotoxicity- based method, in chemico tests, in silico prediction model, and 3D reconstructed human cornealike epithelium (RhCE); (ii) For Draize skin irritation test, in vitro cytotoxicity-based cell model, and 3D reconstructed human epidermis models (RhE). Of these, RhCE and RhE models are getting spotlight as a promising alternative with a wide applicability domain covering cosmetics and personal care products. In this review, we overviewed the current alternatives to Draize test with a focus on 3D human epithelium models to provide an insight into advancing and widening their utility.
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页码：191 / 203
页数：12
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  • [1] Winder C., Azzi R., Wagner D., The development of the globally harmonized system (GHS) of classification and labelling of hazardous chemicals, J. Hazard. Mater, 125, pp. 29-44, (2005)
  • [2] (2002)
  • [3] Wilhelmus K.R., The Draize eye test, Surv. Ophthalmol, 45, pp. 493-515, (2001)
  • [4] York M., Steiling W., A critical review of the assessment of eye irritation potential using the Draize rabbit eye test, J. Appl. Toxicol, 18, pp. 233-240, (1998)
  • [5] Christian M.S., Diener R.M., Soaps and detergents: alternatives to animal eye irritation tests, Int. J. Toxicol, 15, pp. 1-44, (1996)
  • [6] Draize J.H., Woodard G., Calvery H.O., Methods for the study of irritation and toxicity of substances applied topically to the skin and mucous membranes, J. Pharmacol. Exp. Ther, 82, pp. 377-390, (1944)
  • [7] Fitzhugh O., Woodard G., The toxicities of compounds related to 2, 3-dimercaptopropanol (BAL) with a note on their relative therapeutic efficiency, J. Pharmacol. Exp. Ther, 87, pp. 23-27, (1946)
  • [8] Kay J., Calandra J., Interpretation of eye irritation tests, J. Soc. Cosmet. Chem, 13, pp. 281-289, (1962)
  • [9] Rohde B., In vivo eye irritation test methods in Ophthalmic Toxicology, pp. 83-108, (1992)
  • [10] Sekizawa J., Yasuhara K., Suyama Y., Yamanaka S., Tobe M., Nishimura M., A simple method for screening assessment of skin and eye irritation, J. Toxicol. Sci, 19, pp. 25-35, (1994)
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