Evaluation of the environmental conditions for the continuous production of lignin peroxidase by Phanerochaete Chrysosporium in fixed-bed bioreactors

被引：0
作者：
S. Rodríguez Couto
G. Feijoo
M.T. Moreira
J.M. Lema
机构：
[1] University of Santiago de Compostela,Department of Chemical Engineering, Institute of Technology
来源：
Biotechnology Letters | 2002年 / 24卷
关键词：
continuous production; fixed-bed bioreactor; lignin peroxidase; pulsation system;
D O I：
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中图分类号：
学科分类号：
摘要：
A new system to produce lignin peroxidase (LiP) continuously by Phanerochaete chrysosporium is described. A fixed-bed bioreactor with a pulsing device was used as the optimal bioreactor configuration. Addition of veratryl alcohol (1 mM), tryptophan (1 mM), no Mn2+ addition, low glucose addition rate (60–70 mg l−1 h) and an atmosphere of O2 gave maximum LiP activities of 700 U l−1, which are higher than those previously reported.
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页码：791 / 794
页数：3
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