Development of 11 polymorphic microsatellite markers for Xylocarpus granatum (Meliaceae) using next-generation sequencing technology

被引：0

作者：

Yuki Tomizawa

Yoshimi Shinmura

Alison K. S. Wee

Koji Takayama

Takeshi Asakawa

Orlex Baylen Yllano

Severino Garengo Salmo

Erwin Riyanto Ardli

Nguyen Xuan Tung

Norhaslinda Binti Malekal

Sankararamasubramanian Halasya Onrizal

Sarawood Meenakshisundaram

Mohd Nazre Sungkaew

Bayu Bin Saleh

Khin Khin Adjie

Emiko Soe

Noriaki Oguri

Yasuyuki Murakami

Shigeyuki Watano

Edward L. Baba

Tadashi Webb

机构：

[1] Chiba University,Department of Biology, Graduate School of Science

[2] National University of Singapore,Department of Biological Sciences

[3] The University of Tokyo,The University Museum

[4] Adventist University of the Philippines,Biology Department, College of Sciences and Technology

[5] Ateneo de Manila University,Department of Environmental Science, School of Science and Engineering

[6] Jenderal Soedirman University,Faculty of Biology

[7] Hanoi National University of Education,Mangrove Ecosystem Research Centre

[8] Universiti Malaysia Sabah,Institute for Tropical Biology and Conservation

[9] Universitas Sumatera Utara,Forestry Sciences Department

[10] Swaminathan Research Foundation,Biotechnology Programme

[11] Kasesart University,Faculty of Forestry

[12] Universiti Putra Malaysia,Department of Forest Management, Faculty of Forestry

[13] Bali Botanical Garden,Department of Botany

[14] University of Yangon,Department of Biological Sciences

[15] Tokyo Metropolitan University,Tropical Biosphere Research Center

[16] University of the Ryukyus,undefined

来源：

Conservation Genetics Resources | 2013年 / 5卷

关键词：

Conservation; Genetic diversity; Mangrove; Pyrosequencing; SSR; Indo-West Pacific;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Human impacts have seriously damaged mangroves, and conservation of mangroves will require information on local and regional population genetic structures. Here, we report the development and polymorphism of eleven novel microsatellite markers, developed using next-generation sequencing on 56 samples of widespread mangrove species Xylocarpus granatum (Meliaceae) from nine populations across the Indo-West Pacific region. All loci were found to be polymorphic, with the number of alleles per locus ranging from four to 19. In a population from Sabah (Malaysia), the mean observed and expected heterozygosity per locus was 0.59 and 0.58, respectively. No null allele, significant linkage disequilibrium or deviation from Hardy–Weinberg equilibrium was detected among all loci. The eleven markers developed can be valuable tools to conservation genetics of this species across its distributional range.

引用

页码：1159 / 1162

页数：3

共 22 条

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[7] Martin J-F(undefined)undefined undefined undefined undefined-undefined
[8] Raymond M(undefined)undefined undefined undefined undefined-undefined
[9] Rousset F(undefined)undefined undefined undefined undefined-undefined
[10] Shinmura Y(undefined)undefined undefined undefined undefined-undefined

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