Development of 11 polymorphic microsatellite markers for Xylocarpus granatum (Meliaceae) using next-generation sequencing technology

被引:0
作者
Yuki Tomizawa
Yoshimi Shinmura
Alison K. S. Wee
Koji Takayama
Takeshi Asakawa
Orlex Baylen Yllano
Severino Garengo Salmo
Erwin Riyanto Ardli
Nguyen Xuan Tung
Norhaslinda Binti Malekal
Sankararamasubramanian Halasya Onrizal
Sarawood Meenakshisundaram
Mohd Nazre Sungkaew
Bayu Bin Saleh
Khin Khin Adjie
Emiko Soe
Noriaki Oguri
Yasuyuki Murakami
Shigeyuki Watano
Edward L. Baba
Tadashi Webb
机构
[1] Chiba University,Department of Biology, Graduate School of Science
[2] National University of Singapore,Department of Biological Sciences
[3] The University of Tokyo,The University Museum
[4] Adventist University of the Philippines,Biology Department, College of Sciences and Technology
[5] Ateneo de Manila University,Department of Environmental Science, School of Science and Engineering
[6] Jenderal Soedirman University,Faculty of Biology
[7] Hanoi National University of Education,Mangrove Ecosystem Research Centre
[8] Universiti Malaysia Sabah,Institute for Tropical Biology and Conservation
[9] Universitas Sumatera Utara,Forestry Sciences Department
[10] Swaminathan Research Foundation,Biotechnology Programme
[11] Kasesart University,Faculty of Forestry
[12] Universiti Putra Malaysia,Department of Forest Management, Faculty of Forestry
[13] Bali Botanical Garden,Department of Botany
[14] University of Yangon,Department of Biological Sciences
[15] Tokyo Metropolitan University,Tropical Biosphere Research Center
[16] University of the Ryukyus,undefined
来源
Conservation Genetics Resources | 2013年 / 5卷
关键词
Conservation; Genetic diversity; Mangrove; Pyrosequencing; SSR; Indo-West Pacific;
D O I
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中图分类号
学科分类号
摘要
Human impacts have seriously damaged mangroves, and conservation of mangroves will require information on local and regional population genetic structures. Here, we report the development and polymorphism of eleven novel microsatellite markers, developed using next-generation sequencing on 56 samples of widespread mangrove species Xylocarpus granatum (Meliaceae) from nine populations across the Indo-West Pacific region. All loci were found to be polymorphic, with the number of alleles per locus ranging from four to 19. In a population from Sabah (Malaysia), the mean observed and expected heterozygosity per locus was 0.59 and 0.58, respectively. No null allele, significant linkage disequilibrium or deviation from Hardy–Weinberg equilibrium was detected among all loci. The eleven markers developed can be valuable tools to conservation genetics of this species across its distributional range.
引用
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页码:1159 / 1162
页数:3
相关论文
共 22 条
  • [1] Meglécz E(2010)QDD: a user-friendly program to select microsatellite markers and design primers from large sequencing projects Bioinformatics 26 403-404
  • [2] Costedoat C(1995)GENEPOP (Version 1.2): population genetics software for exact tests and ecumenicism J Hered 86 248-249
  • [3] Dubut V(2012)Development and characterization of 15 polymorphic microsatellite loci in Conserv Genet Resour 60 1442-1449
  • [4] Gilles A(2011) (Lythraceae) using next-generation sequencing Taxon 4 535-538
  • [5] Malausa T(2004)A simple and cost-effective approach for microsatellite isolation in non-model plant species using small-scale 454 pyrosequencing Mol Ecol Notes undefined undefined-undefined
  • [6] Pech N(undefined)Micro-checker: software for identifying and correcting genotyping errors in microsatellite data undefined undefined undefined-undefined
  • [7] Martin J-F(undefined)undefined undefined undefined undefined-undefined
  • [8] Raymond M(undefined)undefined undefined undefined undefined-undefined
  • [9] Rousset F(undefined)undefined undefined undefined undefined-undefined
  • [10] Shinmura Y(undefined)undefined undefined undefined undefined-undefined