Influence of tissue-specific superoxide dismutase gene expression in brain cells on Drosophila melanogaster sensitivity to oxidative stress and viability

被引：0
作者：
M. V. Vitushynska
N. P. Matiytsiv
Y. I. Chernyk
机构：
[1] Ivan Franko National University of Lviv,
来源：
Cytology and Genetics | 2015年 / 49卷
关键词：
genes; oxidative stress; prooxidant; life span; and neurodegeneration;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The study has shown that both functional knockout of Sod1 and Sod2 genes encoding superoxide dismutase and their overexpression in neurons and glial tissue increases the sensitivity of Drosophila melanogaster to oxidative stress (OS) conditions. The lowest survival rate was only 20.5% in insects with Sod2 knockout in neurons. The comparative analysis of survival curves showed that adults with altered tissue-specific expression of the studied genes had reduced average and maximum life span. Under OS conditions induced by 5% hydrogen peroxide, the life spans of wild type Oregon R and transgenic insects were significantly reduced. Neurodegenerative phenotype is observed in 3-day-old imago with functional inactivation of Sod genes in glial tissue. During the aging of insects and the action of prooxidants, the increase of neurodegenerative phenotype is detected.
引用
收藏
页码：95 / 101
页数：6
相关论文
共 36 条
  • [1] Boldyrev AA(2001)Oxidative stress and the brain Soros. Obraz. Zh. 7 21-25
  • [2] Perez VI(2009)Is the oxidative stress theory of aging dead? Biohim. Biophys. Acta 1790 1005-1014
  • [3] Bokov A(1995)Superoxide radical and superoxide dismutases Annu. Rev. Biochem. 64 97-112
  • [4] van Remmen H(2009)Regulation of superoxide dismutase genes: implications in disease Free Radic. Biol. Med. 47 344-356
  • [5] Fridovich I(2012)Mutant SOD1 forms ion channel: implications for ALS pathophysiology Neurobiol. Dis. 45 831-838
  • [6] Miao L(1994)Molecular structure and organization of the human manganese superoxide dismutase gene DNA Cell Biol. 13 1127-1136
  • [7] St. Clair DK(2004)Oxidative stress in neurodegeneration: cause or consequence? Nat. Med. 10 18-25
  • [8] Allen MJ(2012)A novel Brain Behav. 2 424-434
  • [9] Lacroix JJ(2011) SOD2 mutant demonstrates a role for mitochondrial ROS in neurodevelopment and disease Biopolym. Cell 27 453-458
  • [10] Capone R(2005)Sensitivity of neurodegenerative mutants of Invert. Neurosci. 5 97-109
1234