Decontamination of heart valve and arterial allografts in the European Homograft Bank (EHB): Comparison of two different antibiotic cocktails in low temperature conditions

被引：32

作者：

Jashari R. ^{[1
]}

Tabaku M. ^{[1
]}

Van Hoeck B. ^{[1
]}

Cochéz C. ^{[1
]}

Callant M. ^{[2
]}

Vanderkelen A. ^{[1
]}

机构：

[1] European Homograft Bank, International Association, Military Hospital, Brussels 1120, Rue Bruynstaat

[2] LCA (Laboratory for Control and Analysis), Brussels

来源：

Cell and Tissue Banking | 2007年 / 8卷 / 4期

关键词：

Allograft; Antibiotic cocktail; Arterial allograft; Bacterial contamination; Cryopreservation; Donor tissue; Heart valve allograft;

D O I：

10.1007/s10561-007-9040-5

中图分类号：

学科分类号：

摘要：

The aim of the study was to compare the efficiency of two different antibiotic cocktails in the cardiovascular allograft decontamination. Low temperature, low-concentration antibiotic cocktail with Cefoxitin, Lincomycin, Polymixin B and Vancomycin was decontamination protocol in EHB for many years. The modified cocktail doesn't contain Cefoxitin. The study had two steps. First step: cardiovascular allografts from 80 donors are incubated in classical (group 1) or modified cocktail (group 2). Second step: 184 and 182 allografts of group 1 and group 2 are incubated in the modified and classical antibiotic cocktail, respectively. The bacteriological examination is performed in three steps: A-transport solution, B-decontamination solution and C-cryopreservation solution. During the first step 23.75% of the tissues were initially contaminated mainly with Staphylococcus (78.95%). 93.75% of the allografts of group 1 and 100% of group 2 were sterile after incubation (p = 0.058). 25.54% and 30.77% of group 1 and 2, respectively were contaminated in A-examination during the second step. Staphylococci were isolated in 82.98% and 69.64% in group 1 and 2, respectively. About 4.35% of group 1 and 5.5% of group 2 were contaminated in A, B, and C whereas 5.4% of group 1 and 4.4% of group 2 were contaminated in B or C after being sterile in A. Finally 9.78% of the tissues were rejected and 90.22% cryopreserved in the modified, whereas 9.89% rejected and 90.11% accepted in the classical group (p = 0.1). The difference was non-significant in the level of decontamination between the two cocktails. Contamination of some tissues with low growing, low-pathogen germs that appeared in B or C examination, couldn't be explained. This issue needs complementary investigation. © Springer Science+Business Media, Inc. 2007.

引用

页码：247 / 255

页数：8

共 29 条

[1]

Barrat-Boyes B.G., Homograft aortic valve replacement in aortic incompetence and stenosis, Thorax, 19, pp. 131-150, (1964)

[2]

Barratt-Boyes B.G., Roche A.H.G., Brandt P.W.T., Et al., Aortic homograft valve replacement: A longterm follow-up of an initial series of 101 patients, Circulation, 40, (1969)

[3]

Brockbank K.G.M., Dawson P.E., Citotoxicity of Amphotericin B for fibroblasts in human heart valve leaflets, Cryobiology, 30, pp. 19-24, (1993)

[4]

Conte S., Jashari R., Et al., Homograft valve insertion for pulmonary regurgitation late after valveless repair of right ventricular outflow tract obstruction, Eur J Cardio-thorac Surg, 15, pp. 143-149, (1999)

[5]

Daene W., Narine K., Goffin Y., Gewillig M., Right ventricular outflow tract reconstruction with homografts, Eur J Cardio-thorac Surg, 9, pp. 448-452, (1995)

[6]

Manual of the Allograft Processing (Heart Valves-Arteries)

[7]

Gall K., Smith S., Et al., Allograft valve sterilisation: A six-year in-depth analysisof a twenty-five year experience with low-dose antibiotics, J Thorac Cardiovasc Surg, 110, pp. 680-687, (1995)

[8]

Gall K.L., Smith S.E., Willmette C.A., O'Brien M.F., Allograft heart valve viability and valve-processing variables, Ann Thorac Surg, 65, pp. 1032-1038, (1997)

[9]

Goffin Y., Van Hoeck B., Jashari R., Soots G., Kalmar P., Banking of cryopreserved heart valves in Europe: Assessment of a 10 year operation in the European Homograft Bank (EHB), J Heart Valve Dis, 9, pp. 207-214, (2000)

[10]

Ireland L., Spelman D., Bacteriological contamination of tissue allografts-experience of the donor tissue bank of Vicoria, Cell Tissue Bank, 6, pp. 181-189, (2005)

← 1 2 3 →