Mechanistic studies of O2-resistant N2-fixation in N2-fixing Escherichia coli

被引：0
作者：
Shuang-Jiang Liu
Hitoshi Iwahashi
Yasuhiko Komatsu
机构：
[1] National Institute of Bioscience and Human-technology,Institute of Microbiology
[2] Chinese Academy of Sciences,undefined
来源：
Biotechnology Letters | 1998年 / 20卷
关键词：
Escherichia Coli; Organic Chemistry; Gene Cluster; Anaerobic Condition; Parent Strain;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Escherichia coli carrying the entire nif gene cluster from Klebsiella pneumoniae on a multicopy plasmid becomes more O2-resistant in a N-free medium as a result of the integration of the nif gene cluster into the chromosome and the loss of the plasmid (H.Iwahashi and J.Someya, Biochem. Biophys. Res. Comm. 1990, 168: 288–294). Our purpose is to characterize the physiological reason why the strain became O2-resistant by measuring the levels of nif proteins in cells under microaerobic conditions. The O2-resistant strain had a higher amount of NifH and a lower amount of NifL under microaerobic conditions (compared to that under anaerobic conditions), while the parent strain showed the opposite characteristics. Thus, the biochemical mechanism of the O2-resistant strain is attributed to the strain's ability to synthesize and maintain a high amount of NifH and a low amount of NifL under microaerobic conditions. © Rapid Science Ltd. 1998
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页码：889 / 893
页数：4
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