The utility of MALDI-TOF MS for outbreak investigation in the neonatal intensive care unit

被引：0
作者：
Maskit Bar-Meir
Elihay Berliner
Livnat Kashat
David A. Zeevi
Marc V. Assous
机构：
[1] Shaare-Zedek Medical Center,Infectious Diseases
[2] Hebrew University,Faculty of Medicine
[3] Shaare-Zedek Medical Center,Pediatrics
[4] Shaare-Zedek Medical Center,The Microbiology Laboratory
[5] Medical Genetics Institute – Shaare-Zedek Medical Center,undefined
来源：
European Journal of Pediatrics | 2020年 / 179卷
关键词：
MALDI-TOF; Neonatal intensive care unit; Whole-genome sequencing; Outbreak; ESBL-producing ;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Our aim was to evaluate the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), routinely used in the microbiology laboratory for bacterial identification, for bacterial typing in the setting of extended spectrum beta-lactamase producing Klebsiella pneumoniae (ESBL-KP) outbreak in the neonatal intensive care unit (NICU). Isolates from a 2011 outbreak in the NICU were retrieved from frozen stocks and analyzed by MALDI-TOF. The MALDI typing was compared with core genome multilocus sequence typing (cg-MLST). MALDI typing divided the 33 outbreak isolates into 2 clones: sequence type (ST)-290 and 405. These results were in complete agreement with cg-MLST results. The differentiation of the outbreak isolates into two clones correlated with the patients’ location in the NICU, but also with their place of residence.
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页码：1843 / 1849
页数：6
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