The effect of zinc(II) on the growth of E . coli studied by microcalorimetry

被引：0
作者：
J. Yao
Y. Liu
H. G. Liang
C. Zhang
J. Z. Zhu
X. Qin
M. Sun
S. S. Qu
Z. N. Yu
机构：
[1] China University of Geosciences,School of Environmental Studies
[2] Wuhan University,Department of Chemistry, College of Chemistry and Molecular Science
[3] Huazhong Agricultural University,Key Laboratory of Agricultural Microbiology of Chinese Agriculture Ministry
来源：
Journal of Thermal Analysis and Calorimetry | 2005年 / 79卷
关键词：
microcalorimetry; E . coli; effect of zinc(II); metabolic action;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
By using an LKB2277 Bioactivity Monitor, stop-flow mode, the power-time curves of E . coli at 37°C effected by zinc(II) were determined. Some parameters, such as growth rate constants k, inhibitory ratio I, the maximum heat production rate Pmax heat output Q and the time in the maximum heat production tmax were obtained. According these parameters, we found that a low concentration of zinc(II) had a promoting action on the growth of E. coli, but a high concentration of zinc(II) had an inhibitory action. The toxicity of zinc(II) can also be expressed as half inhibitory concentration IC50 of zinc(II), i.e., 50% effective in this inhibition. The value of IC50 of zinc(II) on E. coliis 28.09 µg mL-1. The assay is quantitative, inexpensive and versatile.
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页码：39 / 43
页数：4
相关论文
共 44 条
  • [1] Bond J. S.(1983)undefined J. Bio. Chem. 258 8004-undefined
  • [2] Hall D. O.(1996)undefined Acta Physico-Chimica Sinica 12 659-undefined
  • [3] Hawkins S.(2002)undefined J. Therm. Anal. Cal. 70 415-undefined
  • [4] Liu Y.(2000)undefined J. Biochem. Biophys. 40 845-undefined
  • [5] Wang X. Q.(2003)undefined J. Anal. Cal. 72 587-undefined
  • [6] Xie C. L.(1991)undefined Environ. Sci. Technol. 25 1092-undefined
  • [7] Qu S. S.(1986)undefined Microbios 45 7-undefined
  • [8] Yao J.(1982)undefined Chem. Scr 20 155-undefined
  • [9] Liu Y.(2003)undefined J. Therm. Anal. Cal. 71 155-undefined
  • [10] Gao Z. T.(2004)undefined J. Therm. Anal. Cal. 75 293-undefined
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