Characterization of carbonic anhydrase from diversified genus for biomimetic carbon-dioxide sequestration

被引：0
作者：
Anjana Sharma
Abhishek Bhattacharya
Rajesh Pujari
Ankita Shrivastava
机构：
[1] R.D University,Bacteriology Laboratory, Department of P.G studies and Research in Biological Sciences
来源：
Indian Journal of Microbiology | 2008年 / 48卷
关键词：
Carbonic anhydrase; Characterization; CO; sequestration; Optimization;
D O I：
暂无
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学科分类号：
摘要：
Diversified group of bacteria were screened for carbonic anhydrase (CA) activity. Significant CA activity was found in crude enzyme extracts of Enterobacter and Aeromonas isolates while minimal or negligible CA activity was observed in case of Shigella and Klebsiella spp. Optimization and characterization study of potent CA producing isolates revealed that the maximum enzyme activity of 3.86 EU/ml was observed in E. taylorae and the optimum pH range for enzyme stability was found to be 7.5–9.0 along with an optimum temperature range of 35–50 °C. The molecular mass of CA was 29-kDa indicating α-type with periplasmic and cytosolic location. Present investigation for the first time reports CA in diversified genus and optimized parameters for enhanced production of CA in Enterobacter sp. & Aeromonas sp. from fresh water bodies that inturn lay down grounds for exploitation of CA from E. taylorae as an efficient catalyst for CO2 sequestration within a bioreactor.
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