The L-type lectin receptor-like kinase (PnLecRLK1) from the Antarctic moss Pohlia nutans enhances chilling-stress tolerance and abscisic acid sensitivity in Arabidopsis

被引:0
作者
Shenghao Liu
Jing Wang
Kaoshan Chen
Zhaohui Zhang
Pengying Zhang
机构
[1] State Oceanic Administration,Key Laboratory of Marine Bioactive Substance, Marine Ecology Research Center, The First Institute of Oceanography
[2] Shandong University,National Glycoengineering Research Center, School of Life Sciences
来源
Plant Growth Regulation | 2017年 / 81卷
关键词
Abiotic stress; Antarctica; Bryophyte; Protein kinase; Psychrophile;
D O I
暂无
中图分类号
学科分类号
摘要
Lectin receptor-like kinases (LecRLK) are widespread in higher plants and their effects on abiotic stress tolerance are gradually being reported. However, little information is available on LecRLK functions in bryophytes. Here, an L-type LecRLK gene (PnLecRLK1) was characterized from the Antarctic moss Pohlia nutans. Subcellular localization analysis revealed that PnLecRLK1 was a plasma membrane protein. The expression of PnLecRLK1 was rapidly induced by simulated cold, salt, and drought stresses as well as by exogenously applied abscisic acid (ABA) and methyl jasmonate. Transgenic Arabidopsis plants of overexpressing PnLecRLK1 exhibited enhanced tolerance to chilling-stress and increased ABA sensitivity. Additionally, the expression levels of genes in the C-repeat binding factor (CBF) signaling pathway such as AtCBF1, AtCBF2, AtCBF3 and AtCOR47 were markedly increased in transgenic Arabidopsis. Furthermore, the expression levels of ABA-responsive genes, such as AtABI4, AtABI5, AtMYB2 and AtDREB2A, were also significantly up-regulated in transgenic Arabidopsis. Therefore, our results suggested that PnLecRLK1 functions as a membrane-bound regulator that increases chilling stress tolerance and ABA sensitivity to enable P. nutans to adapt to polar climates.
引用
收藏
页码:409 / 418
页数:9
相关论文
共 216 条
[91]  
Shinozaki K(undefined)undefined undefined undefined undefined-undefined
[92]  
Yamaguchi-Shinozaki K(undefined)undefined undefined undefined undefined-undefined
[93]  
Osakabe Y(undefined)undefined undefined undefined undefined-undefined
[94]  
Mizuno S(undefined)undefined undefined undefined undefined-undefined
[95]  
Tanaka H(undefined)undefined undefined undefined undefined-undefined
[96]  
Maruyama K(undefined)undefined undefined undefined undefined-undefined
[97]  
Osakabe K(undefined)undefined undefined undefined undefined-undefined
[98]  
Todaka D(undefined)undefined undefined undefined undefined-undefined
[99]  
Fujita Y(undefined)undefined undefined undefined undefined-undefined
[100]  
Kobayashi M(undefined)undefined undefined undefined undefined-undefined