Monte Carlo simulation of DNA strand-break induction in supercoiled plasmid pBR322 DNA from indirect effects

被引：0

作者：

H. Tomita

M. Kai

T. Kusama

A. Ito

机构：

[1] Department of Radiological Health,

[2] Faculty of Medicine,undefined

[3] University of Tokyo,undefined

[4] 7-3-1 Hongo,undefined

[5] Bunkyo-ku,undefined

[6] Tokyo 113,undefined

[7] Japan,undefined

[8] Department of Physics,undefined

[9] Cancer Institute,undefined

[10] 1-37-1 Kami-Ikebukuro,undefined

[11] Toshima-ku,undefined

[12] Tokyo 170,undefined

[13] Japan,undefined

来源：

Radiation and Environmental Biophysics | 1998年 / 36卷

关键词：

Monte Carlo Simulation; Energy Deposition; Simulation Code; Plasmid pBR322; Track Structure;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

We present a new Monte Carlo simulation code system (DBREAK) of the detailed events that occur when ionizing radiation interacts with water and DNA molecules. The model treats the initial energy deposition by radiation, the formation of chemically active species, subsequent diffusion-controlled chemical reactions, and induction of DNA strand breaks. DBREAK assumes one-hit single-strand break (SSB) and two-hit double-strand break (DSB) mechanisms. A high-resolution model of plasmid DNA structure has been introduced. The calculated results are compared with the results of previously performed experiments of the same type. Under aerobic conditions, 89.4% of the DNA damage was attributed to OH-radical and 10.5% and 0.1% to e–aq and H, respectively. We also compared the differences between liquid-water track structure and gas-phase-water track structure. The calculated yield of SSBs by liquid-water track structure exceeded that of gas-phase-water track structure by a factor of 1.2.

引用

页码：235 / 241

页数：6