Empirical validation of a two-state kinetic model for the B-Z transition of double-stranded poly[d(G-m5C)]

被引：0

作者：

M. A. Fuertes

José Manuel Pérez

Víctor Manuel González

Carlos Alonso

机构：

[1] Centro de Biología Molecular “Severo Ochoa” (CSIC-UAM) Facultad de Ciencias,

[2] Universidad Autónoma de Madrid Cantoblanco,undefined

[3] E-28049 Madrid,undefined

[4] Spain e-mail: mafuertes@trasto.cbm.uam.es,undefined

[5] Tel.: +34-91-3978463,undefined

[6] Fax: +34-91-3974799,undefined

[7] Departamento de Química Inorgánica,undefined

[8] Facultad de Ciencias Universidad Autónoma de Madrid,undefined

[9] Cantoblanco E-28049 Madrid,undefined

[10] Spain,undefined

[11] Department of Pathology,undefined

[12] University of Michigan,undefined

[13] Ann Arbor MI 48109-0668,undefined

[14] USA,undefined

来源：

JBIC Journal of Biological Inorganic Chemistry | 1999年 / 4卷

关键词：

Circular dichroism spectroscopy; Synthetic polynucleotide; Salt dependence; Temperature dependence; Left-handed DNA;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

C)] in terms of the salt concentration, the absolute temperature, and the cooperativity index. The validity of the formula was tested using circular dichroism spectroscopy after variation of the type of salt (NaCl, MgCl2), the salt concentration, and the temperature of the polynucleotide solution. A consequence of the proposed function is that in conditions of high salt there is a predictable salt threshold which determines the particular molecular mechanism of the B-Z transition. The paper also describes the way in which this threshold level is temperature dependent. A detailed comparison of our data with the experimental data found by other authors is given. The function agrees quantitatively with the experiments and explains the contrasting results found in the literature about the influence in the B-Z transition of both the temperature and the polymer size.

引用

页码：759 / 765

页数：6