Suppression of methionine-induced colon injury of young rats by cysteine and N-acetyl-l-cysteine

被引:0
作者
Marija Stojanović
Ljiljana Šćepanović
Dušan Todorović
Dušan Mitrović
Vuk Šćepanović
Radomir Šćepanović
Slobodan Ilić
Teja Šćepanović
Milica Labudović Borović
Živana Milićević
Vesna Dragutinović
Sunčica Borozan
Ivana Lalić
Sanja Despotović
Dragan Djuric
机构
[1] Faculty of Medicine,Institute of Medical Physiology “Richard Burian”
[2] University of Belgrade,Institute for Neurosurgery, Clinical Center of Serbia
[3] University of Belgrade,Institute of Histology and Embryology “Aleksandar Đ. Kostić”
[4] Belgrade University of Defense,Institute of Medical Chemistry
[5] Military Medical Academy,Department of Chemistry
[6] University Children’s Hospital,undefined
[7] University of Belgrade,undefined
[8] Institute of Neonatology,undefined
[9] Faculty of Medicine,undefined
[10] University of Belgrade,undefined
[11] Faculty of Medicine,undefined
[12] University of Belgrade,undefined
[13] Faculty of Veterinary Medicine,undefined
[14] University of Belgrade,undefined
来源
Molecular and Cellular Biochemistry | 2018年 / 440卷
关键词
Methionine; Colon; Morphometry; Oxidative stress; Rat;
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摘要
Changes in the methionine metabolism can cause a state called hyperhomocysteinemia, inducing oxidative stress in the gut. The production of free radicals is important in the colon damage caused by methionine. This study aimed at evaluating the effect of the use of l-cysteine and N-acetyl-l-cysteine on the colon morphometry of young rats treated with methionine. A total number of 32 male rats were distributed in a randomized experimental design in 4 groups: control group treated with saline; methionine group; cysteine + methionine group, and N-acetyl-l-cysteine + methionine group. After 21 days of treatment, rats were sacrificed and the colon samples were taken for histological and biochemical analysis. Methionine load increased depth of crypts, the lamina muscularis mucosae thickness, the mucosal height, and the number of cells in lamina propria (p < 0.01). Combination of methionine with l-cysteine (C group) and with N-acetyl-l-cysteine (N group) reversed methionine effects. Methionine treatment increased the GPx activity and MDA concentration, while l-cysteine and N-acetyl-l-cysteine increased the catalase activity compared to methionine group. It was concluded that the use of l-cysteine and N-acetyl-l-cysteine was beneficial to decrease intestinal mucosal height and oxidative damage when methionine was used in combination with them.
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页码:53 / 64
页数:11
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