Polyinosinic-Polycytidylic Acid Induces the Expression of GRO-α in BEAS-2B Cells

被引：0

作者：

Koji Yamashita

Tadaatsu Imaizumi

Kageaki Taima

Takashi Fujita

Akira Ishikawa

Hidemi Yoshida

Chikara Oyama

Kei Satoh

机构：

[1] University School of Medicine,Department of Vascular Biology, Institute of Brain Science

[2] Hirosaki University School of Medicine,The Second Department of Internal Medicine

[3] Tokyo Metropolitan Institute of Medical Science,Department of Tumor Biology

[4] Hirosaki University School of Medicine,Department of Urology

[5] Hirosaki University School of Medicine,Department of Vascular Biology, Institute of Brain Science

来源：

Inflammation | 2005年 / 29卷

关键词：

poly IC; bronchial epithelial cells; GRO-α; dsRNA; IRF-3; RIG-I;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Growth-related oncogene protein-α (GRO-α)/CXCLl is a chemokine that activates neutrophils and plays an important role in inflammatory reactions. Polyinosinic-polycytidylic acid (poly IC) is a synthetic double-stranded RNA (dsRNA), which is a ligand for Toll-like receptor-3. Poly IC mimics viral infection when applied to cells and induces inflammatory and immune responses. In the present study, we found the induction of GRO-α in BEAS-2B bronchial epithelial cells treated with poly IC. Pretreatment of cells with 2-aminopurine, an inhibitor for dsRNA-dependent protein kinase (PKR), inhibited the expression of GRO-α-induced by poly IC. Overexpression of interferon-regulatory factor-3 (IRF-3) or retinoic-acid inducible gene-I (RIG-I) enhanced the induction of GRO-α by poly IC. PKR, IRF-3, and RIG-I may be involved in the poly IC-induced expression of GRO-α in BEAS-2B cells. Airway viral infection may elicit GRO-α expression in the bronchial epithelium, which may be implicated in inflammatory and immune reactions.

引用

页码：17 / 21

页数：4

共 148 条

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