Differential basal synthesis of Hsp70/Hsc70 contributes to interindividual variation in Hsp70/Hsc70 inducibility

被引：0

作者：

T. Boshoff

F. Lombard

R. Eiselen

J. J. Bornman

M. Bachelet

B. S. Polla

L. Bornman*

机构：

[1] Biochemistry Division,

[2] Department of Chemistry and Biochemistry,undefined

[3] Rand Afrikaans University,undefined

[4] P.O. Box 524,undefined

[5] Auckland Park 2006 (South Africa),undefined

[6] Fax +27 11 489 2605,undefined

[7] e-mail: LBO@na.rau.ac.za,undefined

[8] Department of Statistics,undefined

[9] Rand Afrikaans University,undefined

[10] P.O. Box 524,undefined

[11] Auckland Park 2006 (South Africa),undefined

[12] Statistical Consultation Service,undefined

[13] Rand Afrikaans University,undefined

[14] P.O. Box 524,undefined

[15] Auckland Park 2006 (South Africa),undefined

[16] Kynoch (Pty.) Ltd.,undefined

[17] P.O. Box 3836,undefined

[18] Randburg 2125 (South Africa),undefined

[19] Laboratoire de Physiologie Respiratoire,undefined

[20] UFR Cochin Port-Royal,undefined

[21] 24 rue du Faubourg Saint Jacques,undefined

[22] Paris University V,undefined

[23] 75014 Paris (France),undefined

来源：

Cellular and Molecular Life Sciences CMLS | 2000年 / 57卷

关键词：

Key words. HSP; Hsp70; Hsc70; basal synthesis; induction; population; human monocyte.;

D O I：

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中图分类号：

学科分类号：

摘要：

The source of intraspecies variation in the expression of heat shock proteins (HSPs) remains unresolved but could shed light on differential stress tolerance and disease susceptibility. This study investigated the influence of variable basal HSP synthesis on differential inducibility of HSP synthesis. Basal and heat-induced synthesis of the major HSP families in peripheral blood monocytes from healthy donors (n=42) were analysed using biometabolic labelling and densitometry. Basal Hsp70/Hsc70 synthesis and percentage induction of Hsp70/Hsc70 synthesis were significantly correlated (r=−0.57, p<0.0001), and described most accurately by an exponential decay equation (R=0.68, R2=0.46). This regression equation suggests that increasing levels of basal Hsp70/Hsc70 synthesis are accompanied byan exponential decrease in the percentage induction of Hsp70/Hsc70 synthesis. The model fits data from European and non-European population groups independently, although both coefficients in the regression equation were larger for non-Europeans. This implies population group as an additional factor influencing differential HSP expression. The differential inducibility of Hsp70/Hsc70 due to variable basal synthesis of Hsp70/Hsc70 and based upon population group may contribute to differential stress tolerance or disease susceptibility.

引用

页码：1317 / 1325

页数：8