Fabrication and application of flexible, multimodal light-emitting devices for wireless optogenetics

被引：0
作者：
Jordan G McCall
Tae-il Kim
Gunchul Shin
Xian Huang
Yei Hwan Jung
Ream Al-Hasani
Fiorenzo G Omenetto
Michael R Bruchas
John A Rogers
机构：
[1] Washington University School of Medicine,Department of Anesthesiology, Division of Basic Research
[2] Washington University Pain Center,Division of Biology and Biomedical Sciences
[3] Washington University School of Medicine,Department of Materials Science and Engineering
[4] Anatomy and Neurobiology,Department of Biomedical Engineering
[5] Washington University School of Medicine,Department of Physics
[6] Washington University School of Medicine,Department of Electrical and Computer Engineering
[7] School of Chemical Engineering,Department of Mechanical Science and Engineering
[8] Sungkyunkwan University (SKKU),Department of Chemistry
[9] IBS Center for Neuroscience Imaging Research,undefined
[10] Institute for Basic Science (IBS),undefined
[11] Frederick Seitz Materials Research Laboratory,undefined
[12] University of Illinois at Urbana-Champaign,undefined
[13] Tufts University,undefined
[14] Tufts University,undefined
[15] University of Illinois at Urbana-Champaign,undefined
[16] University of Illinois at Urbana-Champaign,undefined
[17] University of Illinois at Urbana-Champaign,undefined
[18] Present address: Department of Electrical and Computer Engineering,undefined
[19] University of Wisconsin-Madison,undefined
[20] Wisconsin,undefined
[21] USA.,undefined
来源：
Nature Protocols | 2013年 / 8卷
关键词：
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The rise of optogenetics provides unique opportunities to advance materials and biomedical engineering, as well as fundamental understanding in neuroscience. This protocol describes the fabrication of optoelectronic devices for studying intact neural systems. Unlike optogenetic approaches that rely on rigid fiber optics tethered to external light sources, these novel devices carry wirelessly powered microscale, inorganic light-emitting diodes (μ-ILEDs) and multimodal sensors inside the brain. We describe the technical procedures for construction of these devices, their corresponding radiofrequency power scavengers and their implementation in vivo for experimental application. In total, the timeline of the procedure, including device fabrication, implantation and preparation to begin in vivo experimentation, can be completed in ∼3–8 weeks. Implementation of these devices allows for chronic (tested for up to 6 months) wireless optogenetic manipulation of neural circuitry in animals navigating complex natural or home-cage environments, interacting socially, and experiencing other freely moving behaviors.
引用
收藏
页码：2413 / 2428
页数：15
相关论文
共 95 条
  • [1] Zhang F(2010)Optogenetic interrogation of neural circuits: technology for probing mammalian brain structures Nat. Protoc. 5 439-456
  • [2] Yizhar O(2011)Optogenetics in neural systems Neuron 71 9-34
  • [3] Fenno LE(2007)Neural substrates of awakening probed with optogenetic control of hypocretin neurons Nature 450 420-424
  • [4] Davidson TJ(2011)The development and application of optogenetics Annu. Rev. Neurosci. 34 389-412
  • [5] Mogri M(2010)Tuning arousal with optogenetic modulation of locus coeruleus neurons Nat. Neurosci. 13 1526-1533
  • [6] Deisseroth K(2011)Excitatory transmission from the amygdala to nucleus accumbens facilitates reward seeking Nature 475 377-380
  • [7] Adamantidis AR(2013)Distinct extended amygdala circuits for divergent motivational states Nature 496 224-228
  • [8] Zhang F(2013)Diverging neural pathways assemble a behavioural state from separable features in anxiety Nature 496 219-223
  • [9] Aravanis AM(2012)Input-specific control of reward and aversion in the ventral tegmental area Nature 491 212-217
  • [10] Deisseroth K(2011)Amygdala circuitry mediating reversible and bidirectional control of anxiety Nature 471 358-362
12345678910