Streptosporangium lutulentum sp. nov., Streptosporangium fenghuangense sp. nov. and Streptosporangium corydalis sp. nov., three novel actinobacterial species isolated from National Forest Park of Fenghuang Mountain

被引：0
作者：
Baozhu Fang
Hui Liu
Tong Pan
Chongxi Liu
Xuejiao Guan
Hairong He
Kai Yan
Jiansong Li
Wensheng Xiang
Xiangjing Wang
机构：
[1] Chinese Academy of Agricultural Sciences,State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection
[2] Northeast Agricultural University,Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee
来源：
Antonie van Leeuwenhoek | 2016年 / 109卷
关键词：
sp. nov.; sp. nov.; sp. nov.; Polyphasic taxonomy; 16S rRNA gene;
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摘要：
Three novel actinobacteria, designated strains NEAU-FSHN1T, NEAU-hd-3T and NEAU-Y6T, were isolated from a stream base, soil adjacent to the stream and a root of Corydalis yanhusuo L, respectively, collected from Wuchang, Heilongjiang Province, China. A polyphasic study was carried out to establish the taxonomic positions of these strains. The three strains were observed to form scant aerial hyphae that differentiated into spherical spore vesicles. The phylogenetic analysis based on the 16S rRNA gene sequences of strains NEAU-FHSN1T, NEAU-hd-3T and NEAU-Y6T showed that the three novel isolates exhibit 99.2 % (NEAU-FHSN1T/NEAU-hd-3T), 99.2 % (NEAU-FHSN1T/NEAU-Y6T) and 99.7 % (NEAU-hd-3T/NEAU-Y6T) 16S rRNA gene sequence similarities with each other and that they are closely related to strains Streptosporangium shengliense NEAU-GH7T (sequence similarities 98.72, 98.85, 98.99 %), Streptosporangium roseum DSM 43021T (98.65, 98.51, 98.58 %) and Streptosporangium album DSM 43023T (98.41, 98.96, 98.89 %). However, the DNA–DNA hybridization values between strains NEAU-FSHN1T, NEAU-hd-3T and NEAU-Y6T were 61.2 % (NEAU-FSHN1T/NEAU-hd-3T), 63.5 % (NEAU-FHSN1T/NEAU-Y6T) and 65.8 % (NEAU-hd-3T/NEAU-Y6T), and the values between the three strains and their close phylogenetic relatives were also below 70 %. With reference to phenotypic characteristics, phylogenetic data and DNA–DNA hybridization results, the three strains can be distinguished from each other and their close phylogenetic relatives. Thus, strains NEAU-FHSN1T, NEAU-hd-3T and NEAU-Y6T are concluded to represent three novel species of the genus Streptosporangium, for which the names Streptosporangium lutulentum sp. nov., Streptosporangium fenghuangense sp. nov. and Streptosporangium corydalis sp. nov. are proposed. The type strains are NEAU-FHSN1T (=CGMCC 4.7141T = DSM 46740T), NEAU-Y6T (=CGMCC 4.7150T = DSM 46722T) and NEAU-hd3T (CGMCC 4.7212T = JCM 30058T), respectively.
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页码：439 / 448
页数：9
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共 150 条
  • [1] Couch JN(1955)A new genus and family of the J Elisha Mitchell Sci Soc 71 148-155
  • [2] De Ley J(1970), with a revision of the genus Eur J Biochem 12 133-142
  • [3] Cattoir H(1981)The quantitative measurement of DNA hybridization from renaturation rates J Mol Evol 17 368-376
  • [4] Reynaerts A(1985)Evolutionary trees from DNA sequences: a maximum likelihood approach Evolution 39 783-791
  • [5] Felsenstein J(2014)Confidence limits on phylogenies: an approach using the bootstrap Antonie Van Leeuwenhoek 105 307-315
  • [6] Felsenstein J(1974) sp. nov., isolated from muddy soil in China and emended description of the genus Int J Syst Bacteriol 24 54-63
  • [7] Gao RX(1987), J Ferment Technol 65 501-509
  • [8] Liu CX(2014), and the nocardin strain Antonie Van Leeuwenhoek 105 1025-1031
  • [9] Zhao JW(1983)Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes Syst Appl Microbiol 4 184-192
  • [10] Jia FY(2014) sp. nov., a novel actinomycete isolated from soil Int J Syst Evol Microbiol 64 2400-2406
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